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  Compaction of RNA duplexes in the cell

Collauto, A., von Bülow, S., Gophane, D., Saha, S., Stelzl, L. S., Hummer, G., et al. (2020). Compaction of RNA duplexes in the cell. Angewandte Chemie, International Edition in English, 59, 23025-23029. doi:10.1002/anie.202009800.

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 Creators:
Collauto, Alberto1, Author
von Bülow, Sören2, Author              
Gophane, Dnyaneshwar3, Author
Saha, Subham3, Author
Stelzl, Lukas S.2, Author              
Hummer, Gerhard2, 4, Author              
Sigurdsson, Snorri Th.3, Author
Prisner, Thomas1, Author
Affiliations:
1Institute of Physical and Theoretical Chemistry and Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Frankfurt am Main, Germany, ou_persistent22              
2Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society, ou_2068292              
3Department of Chemistry, Science Institute, University of Iceland, Reykjavik, Iceland, ou_persistent22              
4Institute for Biophysics, Goethe University Frankfurt, Frankfurt am Main, Germany, ou_persistent22              

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Free keywords: EPR spectroscopy, Molecular dynamics, PELDOR/DEER spectroscopy, RNA structures, Site-directed spin labeling
 Abstract: The structure and flexibility of RNA depend sensitively on the microenvironment. Using pulsed electron-electron double-resonance (PELDOR) spectroscopy combined with advanced labeling techniques, we show that the structure of double-stranded RNA (dsRNA) changes upon internalization into Xenopus laevis oocytes. Compared to dilute solution, the dsRNA A-helix is more compact in cells. We recapitulate this compaction in a densely crowded protein solution. Atomic-resolution molecular dynamics simulations of dsRNA capture semi-quantitatively the compaction, and identify non-specific electrostatic interactions between proteins and dsRNA as a possible driver of this effect.

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Language(s): eng - English
 Dates: 2020-07-1620202020-08-172020-12-14
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202009800
BibTex Citekey: collauto_compaction_2020
 Degree: -

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Title: Angewandte Chemie, International Edition in English
  Abbreviation : Angew. Chem., Int. Ed. Engl.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 59 Sequence Number: - Start / End Page: 23025 - 23029 Identifier: ISSN: 0570-0833
CoNE: https://pure.mpg.de/cone/journals/resource/0570-0833