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  Structural base for the transfer of GPI-anchored glycoproteins into fungal cell walls

Vogt, M. S., Schmitz, G. F., Varón Silva, D., Mösch, H.-U., & Essen, L.-O. (2020). Structural base for the transfer of GPI-anchored glycoproteins into fungal cell walls. Proceedings of the National Academy of Sciences of the United States of America, 117(36), 22061-22067. doi:10.1073/pnas.2010661117.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-03E3-5 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-03E4-4
Genre: Journal Article

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 Creators:
Vogt, Marian Samuel, Author
Schmitz, Gesa Felicitas, Author
Varón Silva, Daniel1, Author              
Mösch, Hans-Ulrich, Author
Essen, Lars-Oliver, Author
Affiliations:
1Daniel Varón Silva, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863302              

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Free keywords: fungal cell-wall biogenesis; GPI anchor; glycoside hydrolase; protein trafficking
 Abstract: The correct distribution and trafficking of proteins are essential for all organisms. Eukaryotes evolved a sophisticated trafficking system which allows proteins to reach their destination within highly compartmentalized cells. One eukaryotic hallmark is the attachment of a glycosylphosphatidylinositol (GPI) anchor to C-terminal ω-peptides, which are used as a zip code to guide a subset of membrane-anchored proteins through the secretory pathway to the plasma membrane. In fungi, the final destination of many GPIanchored proteins is their outermost compartment, the cell wall. Enzymes of the Dfg5 subfamily catalyze the essential transfer of GPI-anchored substrates from the plasma membrane to the cell wall and discriminate between plasma membrane-resident GPIanchored proteins and those transferred to the cell wall (GPI-CWP). We solved the structure of Dfg5 from a filamentous fungus and used in crystallo glycan fragment screening to reassemble the GPIcore glycan in a U-shaped conformation within its binding pocket. The resulting model of the membrane-bound Dfg5•GPI-CWP complex is validated by molecular dynamics (MD) simulations and in vivo mutants in yeast. The latter show that impaired transfer of GPI-CWPs causes distorted cell-wall integrity as indicated by increased chitin levels. The structure of a Dfg5•β1,3-glycoside complex predicts transfer of GPI-CWP toward the nonreducing ends of acceptor glycans in the cell wall. In addition to our molecular model for Dfg5-mediated transglycosylation, we provide a rationale for how GPI-CWPs are specifically sorted toward the cell wall by using GPI-core glycan modifications.

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Language(s): eng - English
 Dates: 2020-08-242020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1073/pnas.2010661117
BibTex Citekey: Vogt202010661
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 117 (36) Sequence Number: - Start / End Page: 22061 - 22067 Identifier: ISSN: 0027-8424