English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Underground isoleucine biosynthesis pathways in E. coli

Cotton, C. A. R., Bernhardsgrütter, I., He, H., Burgener, S., Schulz, L., Paczia, N., et al. (2020). Underground isoleucine biosynthesis pathways in E. coli. eLife, 9: e54207. doi:10.7554/eLife.54207.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0007-0058-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-0059-5
Genre: Journal Article

Files

show Files

Locators

show
hide
Locator:
Link (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Cotton, C. A. R.1, Author              
Bernhardsgrütter, Iria2, Author
He, H.1, Author              
Burgener, Simon2, Author
Schulz, Luca2, Author
Paczia, Nicole2, Author
Dronsella, B.1, Author              
Erban, A.3, Author              
Toman, St.1, Author              
Dempfle, M.1, Author              
de Maria, A.1, Author              
Kopka, J.3, Author              
Lindner, S. N.1, Author              
Erb, Tobias J2, Author
Bar-Even, A.1, Author              
Affiliations:
1Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2035297              
2External Organizations, ou_persistent22              
3Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

Content

show
hide
Free keywords: -
 Abstract: The promiscuous activities of enzymes provide fertile ground for the evolution of new metabolic pathways. Here, we systematically explore the ability of ̑extitE. coli} to harness underground metabolism to compensate for the deletion of an essential biosynthetic pathway. By deleting all threonine deaminases, we generated a strain in which isoleucine biosynthesis was interrupted at the level of 2-ketobutyrate. Incubation of this strain under aerobic conditions resulted in the emergence of a novel 2-ketobutyrate biosynthesis pathway based upon the promiscuous cleavage of ̑extit{O-succinyl-L-homoserine by cystathionine γ-synthase (MetB). Under anaerobic conditions, pyruvate formate-lyase enabled 2-ketobutyrate biosynthesis from propionyl-CoA and formate. Surprisingly, we found this anaerobic route to provide a substantial fraction of isoleucine in a WT strain, when propionate is available in the medium. This study demonstrates the selective advantage underground metabolism offers, providing metabolic redundancy and flexibility which allow for the best use of environmental carbon sources.

Details

show
hide
Language(s): eng - English
 Dates: 2020-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.7554/eLife.54207
BibTex Citekey: 10.7554/eLife.54207
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: eLife
Source Genre: Journal
 Creator(s):
Chaiyen, Pimchai, Editor
Affiliations:
-
Publ. Info: Cambridge : eLife Sciences Publications
Pages: - Volume / Issue: 9 Sequence Number: e54207 Start / End Page: - Identifier: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X