English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Fine-scale analysis of mechanisms and controlling factors in a meiotic recombination hotspot in dogs (canis familiaris)

Jeschke, A. (2020). Fine-scale analysis of mechanisms and controlling factors in a meiotic recombination hotspot in dogs (canis familiaris). PhD Thesis, Christian Albrecht University of Kiel, Kiel.

Item is

Files

show Files
hide Files
:
Online_Thesis_final.pdf (Publisher version), 18MB
 
File Permalink:
-
Name:
Online_Thesis_final.pdf
Description:
-
OA-Status:
Visibility:
Private
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Jeschke, Alina1, 2, Author           
Odenthal-Hesse, Linda2, Advisor           
Tautz, Diethard3, Referee           
Stukenbrock, Eva H.4, Referee           
Affiliations:
1IMPRS for Evolutionary Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445639              
2Research Group Meiotic Recombination and Genome Instability, Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2355693              
3Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
4Max Planck Fellow Group Environmental Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2068284              

Content

show
hide
Free keywords: -
 Abstract: Meiotic recombination re-shuffles genomes from one generation to the next. In humans and most other mammals, meiotic recombination events are clustered in 1-2 kb wide recombination hotspots, whose locations are determined in trans by the protein PR-domain containing 9 (PRDM9). Mice lacking PRDM9 direct recombination to promoters and functional elements, resulting in meiotic defects. Dogs (Canis familiaris) lack a functional copy of PRDM9, yet linkage data showed that historical recombination events cluster in functional elements, suggesting that there may be a mechanism enabling controlled recombination at these locations, and in the absence of PRDM9. However nothing is known about the de-novo activity of dog recombination hotspots and the patters of recombination resolution in this PRDM9 deficient species. I investigated a dog recombination hotspot for de-novo recombination events using pooled sperm typing, and uncovered high crossover frequencies affecting up to 1 % of sperm. Frequencies can differ by one order of magnitude between dogs. Fine-scale analysis of crossover-breakpoints revealed wide distributions of breaks across up to 10 kb within the hotspot region. I further detect asymmetric breakpoint distributions between crossover orientations and crossover-associated transmission distortion, suggesting biased recombination-initiation or -repair. This work is an elaborate fine-scale dissection of a mammalian PRDM9-independent active recombination hotspot.

Details

show
hide
Language(s): eng - English
 Dates: 2020-022020-05-202020-05-20
 Publication Status: Published in print
 Pages: 164
 Publishing info: Kiel : Christian Albrecht University of Kiel
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: Diss/13309
 Degree: PhD

Event

show

Legal Case

show

Project information

show

Source

show