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  1H MAS NMR of gramicidin A in a liquid-crystalline multilamellar phase

Farès, C., Davis, J. H., & Sharom, F. J. (1997). 1H MAS NMR of gramicidin A in a liquid-crystalline multilamellar phase. Poster presented at Forty-First Annual Meeting, Biophysical Society, New Orleans, Louisianna, USA.

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 Creators:
Farès, Christophe1, Author              
Davis, James H.1, Author
Sharom, Frances J., Author
Affiliations:
1University of Guelph, Guelph, Ontario, N1G 2W1, Canada, ou_persistent22              

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 Abstract: Using a recently-built very high speed MAS probe capable of spinning at rates of at least 20 kHz, we have shown that it is possible to obtain 1H signals with line widths in the range of 25-30 Hz, an improvement of three orders of magnitude. To establish that it is possible to obtain distance restraints between nuclei, we have performed 1D nuclear Overhauser effect (NOE) experiments on gramicidin A. The build-up curves for these NOEs clearly show that one can obtain the same sort of distance restraints for peptides in membrane bilayers as are obtained in well established solution NMR studies. This is a crucial step in demonstrating that high resolution solution NMR techniques can be applied to structure determination in membrane systems. Extending this to two dimensions, we have performed a series of 2D-NOESY experiments which establish unambiguously that gramicidin A adopts the same conformation in muitilamellar dispersions as that reported in SDS micelles by Arseniev et al. [Biol. Membr. (1986) 3(5), 437-462]. The 1H MAS NMR technique that we have developed is therefore potentially capable of determining the complete 3D structure of a membrane spanning peptide.

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Language(s): eng - English
 Dates: 1997-02-01
 Publication Status: Published in print
 Pages: 1
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 Table of Contents: -
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Title: Forty-First Annual Meeting, Biophysical Society
Place of Event: New Orleans, Louisianna, USA
Start-/End Date: 1997-03-02 - 1997-03-06

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Title: Biophysical Journal (Annual Meeting Abstracts)
  Other : Biophys. J. (Annual Meeting Abstracts)
Source Genre: Journal
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Publ. Info: Bethesda, MD : Biophysical Society
Pages: - Volume / Issue: 72 (2, Part 2) Sequence Number: M-Pos285 Start / End Page: A75 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117_1