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  ICEs are the main reservoirs of the Ciprofloxacin-Modifying crpP gene in Pseudomonas aeruginosa

Botelho, J., Grosso, F., & Peixe, L. (2020). ICEs are the main reservoirs of the Ciprofloxacin-Modifying crpP gene in Pseudomonas aeruginosa. Genes, 11(8): 889. doi:10.3390/genes11080889.

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genes-11-00889-v2.pdf (Publisher version), 3MB
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Botelho, João1, Author           
Grosso, Filipa, Author
Peixe, Luísa, Author
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1Max Planck Fellow Group Antibiotic Resistance Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2600692              

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Free keywords: integrative and conjugative elements; Pseudomonas aeruginosa; ciprofloxacin resistance; crpP; pangenome
 Abstract: The ciprofloxacin-modifying crpP gene was recently identified in a plasmid isolated from a Pseudomonas aeruginosa clinical isolate. Homologues of this gene were also identified in Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii. We set out to explore the mobile elements involved in the acquisition and spread of this gene in publicly available and complete genomes of Pseudomonas spp. All Pseudomonas complete genomes were downloaded from NCBIrsquo;s Refseq library and were inspected for the presence of the crpP gene. The mobile elements carrying this gene were further characterized. The crpP gene was identified only in P. aeruginosa, in more than half of the complete chromosomes (61.9, n = 133/215) belonging to 52 sequence types, of which the high-risk clone ST111 was the most frequent. We identified 136 crpP-harboring integrative and conjugative elements (ICEs), with 93.4 belonging to the mating-pair formation G (MPFG) family. The ICEs were integrated at the end of a tRNALys gene and were all flanked by highly conserved 45-bp direct repeats. The crpP-carrying ICEs contain 26 core genes (2.2 of all 1193 genes found in all the ICEs together), which are present in 99 or more of the crpP-harboring ICEs. The most frequently encoded traits on these ICEs include replication, transcription, intracellular trafficking and cell motility. Our work suggests that ICEs are the main vectors promoting the dissemination of the ciprofloxacin-modifying crpP gene in P. aeruginosa.

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Language(s): eng - English
 Dates: 2020-07-252020-07-012020-07-312020-08-042020
 Publication Status: Published in print
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 Identifiers: DOI: 10.3390/genes11080889
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Title: Genes
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 11 (8) Sequence Number: 889 Start / End Page: - Identifier: ISSN: 2073-4425
CoNE: https://pure.mpg.de/cone/journals/resource/2073-4425