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  The Photocycle of Bacteriorhodopsin in the Two-Dimensional Orthorombic Crystal Form of Purple Membrane

Korenstein, R., Michel, H., & Hess, B. (1985). The Photocycle of Bacteriorhodopsin in the Two-Dimensional Orthorombic Crystal Form of Purple Membrane. Biophysical Journal, 47(3), 295-302. doi:10.1016/S0006-3495(85)83919-9.

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 Creators:
Korenstein, Rafi1, Author
Michel, Hartmut2, Author              
Hess, Benno3, Author              
Affiliations:
1Department of Membrane Research, Weizmann Institute, Rehovot, Israel, ou_persistent22              
2Max Planck Institute of Biochemistry, Max Planck Society, ou_1565141              
3Max-Planck-Institut für Ernährungsphysiologie, Dortmund, Germany, ou_persistent22              

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 Abstract: Laser flash photolysis and low-temperature absorption studies of the photocycle of orthorhombic purple membrane (o-PM) reveal the existence of the same K, L, and M intermediates as found in the native hexagonal purple membrane (h-PM). However, the 0 intermediate is missing in the o-PM. The absorption spectrum of the K intermediate of o-PM is blueshifted by ∼15 nm relative to the K intermediate found in the hexagonal purple membrane. The decay relaxation time constants of M in the o-PM are higher by more than an order of magnitude than the corresponding relaxation time constants in the h-PM. Similarly to the h-PM, the decay of M depends on the pulse width of excitation. The time-independent anisotropy factor obtained in photoselection studies of the M intermediate demonstrates the complete immobility of bacteriorhodopsin (bR) within the o-PM matrix. The same anisotropy factor of 0.3 obtained for o-PM and for h-PM suggests that in both crystalline lattices the transition moment of the retinal chromophore has similar angles with the plane of the membrane. The dependence of the decay kinetics of M on its occupancy may suggest the existence of kinetic coupling between neighboring bR molecules

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Language(s): eng - English
 Dates: 2009-01-061985-03
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0006-3495(85)83919-9
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 47 (3) Sequence Number: - Start / End Page: 295 - 302 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117