Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Prototypical pacemaker neurons interact with the resident microbiota

Klimovich, A., Giacomello, S., Björklund, Å., Faure, L., Kaucka, M., Giez, C., et al. (2020). Prototypical pacemaker neurons interact with the resident microbiota. Proceedings of the National Academy of Sciences of the United States of America, 117(30), 17854-17863. doi:10.1073/pnas.1920469117.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
Link (Verlagsversion)
Beschreibung:
-
OA-Status:
Keine Angabe
Beschreibung:
-
OA-Status:
Grün
Beschreibung:
-
OA-Status:
Sonstiges
externe Referenz:
https://data.mendeley.com/datasets/ctdfn57ds2/2 (Forschungsdaten)
Beschreibung:
-
OA-Status:
Sonstiges

Urheber

einblenden:
ausblenden:
 Urheber:
Klimovich, Alexander, Autor
Giacomello, Stefania, Autor
Björklund, Åsa, Autor
Faure, Louis, Autor
Kaucka, Marketa1, Autor                 
Giez, Christoph, Autor
Murillo-Rincón, Andrea P.1, Autor           
Matt, Ann-Sophie, Autor
Willoweit-Ohl, Doris, Autor
Crupi, Gabriele, Autor
de Anda, Jaime, Autor
Wong, Gerard C. L., Autor
D’Amato, Mauro, Autor
Adameyko, Igor, Autor
Bosch, Thomas C. G., Autor
Affiliations:
1Max Planck Research Group Evolutionary Developmental Dynamics (Kaucká), Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_3164874              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Here, we discover prototypical pacemaker neurons in the ancient cnidarian Hydra and provide evidence for a direct interaction of these neurons with the commensal microbiota. We uncover a remarkable gene-expression program conservation between the Hydra pacemaker neurons and pacemaker cells in Caenorhabditis elegans and the mammalian gut. We suggest that prototypical pacemaker cells emerged as neurons using components of innate immunity to interact with the microbial environment and ion channels to generate rhythmic contractions. The communication of pacemaker neurons with the microbiota represents a mechanistic link between the gut microbiota and gut motility. Our discoveries improve the understanding of the archetypical properties of the enteric nervous systems, which are perturbed in human dysmotility-related conditions.Pacemaker neurons exert control over neuronal circuit function by their intrinsic ability to generate rhythmic bursts of action potential. Recent work has identified rhythmic gut contractions in human, mice, and hydra to be dependent on both neurons and the resident microbiota. However, little is known about the evolutionary origin of these neurons and their interaction with microbes. In this study, we identified and functionally characterized prototypical ANO/SCN/TRPM ion channel-expressing pacemaker cells in the basal metazoan Hydra by using a combination of single-cell transcriptomics, immunochemistry, and functional experiments. Unexpectedly, these prototypical pacemaker neurons express a rich set of immune-related genes mediating their interaction with the microbial environment. Furthermore, functional experiments gave a strong support to a model of the evolutionary emergence of pacemaker cells as neurons using components of innate immunity to interact with the microbial environment and ion channels to generate rhythmic contractions.scRNA-seq data generated in this study have been deposited on the National Center for Biotechnology Information Sequence Read Archive database under the BioProject accession codes PRJNA614614 and PRJNA614611. Expression count matrices, corresponding metadata, and analytical pipelines are available as Mendeley dataset DOI: 1017632/ctdfn57ds2.1. Custom scripts are available at https://github.com/stefaniagiacomello/Hydra_scRNA-seq.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2019-11-202020-06-032020-07-092020-07
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1073/pnas.1920469117
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Proceedings of the National Academy of Sciences of the United States of America
  Andere : Proc. Acad. Sci. USA
  Andere : Proc. Acad. Sci. U.S.A.
  Andere : Proceedings of the National Academy of Sciences of the USA
  Kurztitel : PNAS
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, D.C. : National Academy of Sciences
Seiten: - Band / Heft: 117 (30) Artikelnummer: - Start- / Endseite: 17854 - 17863 Identifikator: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230