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  Modeling membrane morphological change during autophagosome formation

Sakai, Y., Koyama-Honda, I., Tachikawa, M., Knorr, R. L., & Mizushima, N. (2020). Modeling membrane morphological change during autophagosome formation. iScience, 23(9): 101466. doi:10.1016/j.isci.2020.101466.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-05F4-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-4AAD-4
Genre: Journal Article

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 Creators:
Sakai, Yuji, Author
Koyama-Honda, Ikuko, Author
Tachikawa, Masashi, Author
Knorr, Roland L.1, Author              
Mizushima, Noboru, Author
Affiliations:
1Roland Knorr, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288692              

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Free keywords: Biophysics, Cell Biology, Molecular Biology, Membrane Architecture
 Abstract: Summary Autophagy is an intracellular degradation process that is mediated by de novo formation of autophagosomes. Autophagosome formation involves dynamic morphological changes; a disk-shaped membrane cisterna grows, bends to become a cup-shaped structure, and finally develops into a spherical autophagosome. We have constructed a theoretical model that integrates the membrane morphological change and entropic partitioning of putative curvature generators, which we have used to investigate the autophagosome formation process quantitatively. We show that the membrane curvature and the distribution of the curvature generators stabilize disk- and cup-shaped intermediate structures during autophagosome formation, which is quantitatively consistent with in vivo observations. These results suggest that various autophagy proteins with membrane curvature-sensing properties control morphological change by stabilizing these intermediate structures. Our model provides a framework for understanding autophagosome formation.

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Language(s): eng - English
 Dates: 2020-08-152020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.isci.2020.101466
BibTex Citekey: SAKAI2020101466
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Title: iScience
Source Genre: Journal
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Publ. Info: Amsterdam : Cell Press
Pages: - Volume / Issue: 23 (9) Sequence Number: 101466 Start / End Page: - Identifier: ISSN: 2589-0042