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  Characterization of an improved reaction center preparation from the photosynthetic green sulfur bacterium Chlorobium containing the iron-sulfur centers FA and FB and a bound cytochrome subunit

Feiler, U., Nitschke, W., & Michel, H. (1992). Characterization of an improved reaction center preparation from the photosynthetic green sulfur bacterium Chlorobium containing the iron-sulfur centers FA and FB and a bound cytochrome subunit. Biochemistry, 31(9), 2608-2614. doi:10.1021/bi00124a022.

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アイテムのパーマリンク: https://hdl.handle.net/21.11116/0000-0006-FC28-2 版のパーマリンク: https://hdl.handle.net/21.11116/0000-000B-A282-A
資料種別: 学術論文

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 作成者:
Feiler, Ute1, 著者           
Nitschke, Wolfgang2, 著者
Michel, Hartmut1, 著者                 
所属:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              
2Departement de Biologie, Service de Biophysique, CE Saclay, F-91191 Gif sur Yvette Cedex, France , ou_persistent22              

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 要旨: A photosynthetic reaction center complex was prepared from the green sulfur bacterium Chlorobium by solubilization of chlorosome-depleted membranes with lauryl maltoside, followed by anion-exchange chromatography and molecular sieve chromatography. The purified complex was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, optical spectroscopy, and EPR spectroscopy. The major bands migrated at apparent molecular masses of 50, 42, and 32 kDa (heme-staining) and additional weaker bands at 22, 15, and 12 kDa. The isolated reaction center complex contained about 40 bacteriochlorophyll alpha molecules per primary electron donor, P840, assayed by photooxidation. It was competent in stable low-temperature photoreduction of the FeS centers FA and FB. The spectra of these acceptors and their low-temperature photochemistry in the purified complex were the same as found in intact Chlorobium membranes and similar to what had been described for photosystem I from plants. Membrane-bound cytochrome c553 copurified with the reaction center complex. A ratio of about four hemes per P840 was determined. This result indicates that cytochrome c553 that is closely associated with the reaction center is a tetraheme cytochrome, as described for some purple bacteria.

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言語: eng - English
 日付: 1991-12-211991-10-151992-03-10
 出版の状態: 出版
 ページ: 7
 出版情報: -
 目次: -
 査読: 査読あり
 識別子(DOI, ISBNなど): DOI: 10.1021/bi00124a022
 学位: -

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出版物名: Biochemistry
種別: 学術雑誌
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出版社, 出版地: Columbus, Ohio : American Chemical Society
ページ: - 巻号: 31 (9) 通巻号: - 開始・終了ページ: 2608 - 2614 識別子(ISBN, ISSN, DOIなど): ISSN: 0006-2960
CoNE: https://pure.mpg.de/cone/journals/resource/954925384103