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  Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase

Ostermeier, C., Iwata, S., Ludwig, B., & Michel, H. (1995). Fv fragment-mediated crystallization of the membrane protein bacterial cytochrome c oxidase. Nature Structural and Molecular Biology, 2(10), 842-846. doi:10.1038/nsb1095-842.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0006-FC2C-E Version Permalink: https://hdl.handle.net/21.11116/0000-0007-CD40-A
Genre: Journal Article

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 Creators:
Ostermeier, Christian1, Author           
Iwata, So1, Author           
Ludwig, Bernd2, Author
Michel, Hartmut1, Author           
Affiliations:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              
2Johann Wolfgang Goethe-Universität, Biozentrum N200, Institut für Biochemie, Molekulare Genetik, 60439, Frankfurt/M., Germany, ou_persistent22              

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 Abstract: Crystallization of membrane proteins, a prerequisite for their X-ray crystallographic analysis, remains difficult. Here, we demonstrate that the crystallization of the cytochrome c oxidase from Paracoccus denitrificans can be mediated by co-crystallization with an antibody Fv fragment. The crystals obtained contain all four subunits of this membrane protein complex and the Fv fragment. The approach of co-crystallizing membrane proteins with antibody fragments should be useful in obtaining well-ordered crystals of membrane proteins in general.

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Language(s): eng - English
 Dates: 1995-08-141995-08-231995-10-011995-10-01
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nsb1095-842
PMID: 7552705
 Degree: -

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Title: Nature Structural and Molecular Biology
  Other : Nature Struct Biol
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 2 (10) Sequence Number: - Start / End Page: 842 - 846 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763