English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Luminal particles within cellular microtubules

Garvalov, B. K., Zuber, B., Bouchet-Marquis, C., Kudryashev, M., Gruska, M., Beck, M., et al. (2006). Luminal particles within cellular microtubules. The Journal of Cell Biology, 174(6), 759-765. doi:10.1083/jcb.200606074.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Garvalov, Boyan K.1, Author
Zuber, Benoît2, Author
Bouchet-Marquis, Cédric2, Author
Kudryashev, Misha3, Author                 
Gruska, Manuela4, Author
Beck, Martin4, Author           
Leis, Andrew4, Author
Frischknecht, Friedrich3, Author
Bradke, Frank1, Author
Baumeister, Wolfgang4, Author
Dubochet, Jacques2, Author
Cyrklaff, Marek4, Author
Affiliations:
1Max Planck Research Group: Axonal Growth and Regeneration / Bradke, MPI of Neurobiology, Max Planck Society, ou_1113553              
2Laboratory for Ultrastructural Analysis, University of Lausanne, CH-1015 Lausanne, Switzerland, ou_persistent22              
3Parasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany, ou_persistent22              
4Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

Content

show
hide
Free keywords: -
 Abstract: The regulation of microtubule dynamics is attributed to microtubule-associated proteins that bind to the microtubule outer surface, but little is known about cellular components that may associate with the internal side of microtubules. We used cryoelectron tomography to investigate in a quantitative manner the three dimensional structure of microtubules in intact mammalian cells. We show that the lumen of microtubules in this native state is filled with discrete, globular particles with a diameter of 7 nm and spacings between 8 and 20 nm in neuronal cells. Cross-sectional views of microtubules confirm the presence of luminal material in vitreous sections of brain tissue. Most of the luminal particles had connections to the microtubule wall, as revealed in tomograms. A higher accumulation of particles was seen near the retracting plus ends of microtubules. The luminal particles were abundant in neurons, but were also observed in other cells, such as astrocytes and stem cells.

Details

show
hide
Language(s): eng - English
 Dates: 2006-06-142006-08-082006-09-052006-09-11
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1083/jcb.200606074
ISI: 000240697100005
PMID: 16954350
PMC: PMC2064330
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Journal of Cell Biology
  Other : JBC
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 174 (6) Sequence Number: - Start / End Page: 759 - 765 Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024_2