English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Heterogeneity of the osteocyte lacuno-canalicular network architecture and material characteristics across different tissue types in healing bone

Schemenz, V., Gjardy, R. A., Chamasemani, F., Roschger, A., Roschger, P., Zaslansky, P., et al. (2020). Heterogeneity of the osteocyte lacuno-canalicular network architecture and material characteristics across different tissue types in healing bone. Journal of Structural Biology, 212(2): 107616. doi:10.1016/j.jsb.2020.107616.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0007-06F2-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-1550-7
Genre: Journal Article

Files

show Files
hide Files
:
Article.pdf (Publisher version), 12MB
Name:
Article.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Schemenz, Victoria1, Author              
Gjardy, Ralph André2, Author              
Chamasemani, F. , Author
Roschger, Andreas2, Author              
Roschger, P., Author
Zaslansky, P., Author
Helfen, L., Author
Burghammer, M., Author
Fratzl, Peter3, Author              
Weinkamer, Richard1, Author              
Brunner, R., Author
Willie, B., Author
Wagermaier, Wolfgang2, Author              
Affiliations:
1Richard Weinkamer, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863295              
2Wolfgang Wagermaier, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863296              
3Peter Fratzl, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863294              

Content

show
hide
Free keywords: endochondral ossification, lacuno-canalicular network, qBEI, CLSM, SAXS, µCT
 Abstract: Various tissue types, including fibrous connective tissue, bone marrow, cartilage, woven and lamellar bone coexist in healing bone. Similar to all bone tissue type, healing bone contains a lacuno-canalicular network (LCN) housing osteocytes that are known to orchestrate bone remodeling in healthy bone by sensing mechanical strains and translating them into biochemical signals. The structure of the LCN is also hypothesized to influence mineralization processes. Hence, the aim of the present study was to visualize and correlate spatial variations in the LCN topology with mineral characteristics, within and at the interfaces of the different tissue types that comprise healing bone. We applied a correlative multi-method approach to visualize the LCN architecture and quantify mineral particle size and orientation within healing femoral bone in a mouse osteotomy model (26 weeks old C57BL/6 mice). This approach revealed structural differences across several length scales during endochondral ossification within the following regions: calcified cartilage, bony callus, cortical bone and the transition zone between the cortical region and callus that developed during 21 days after the osteotomy. In this transition zone, we observed a continuous convergence of mineral characteristics and osteocyte lacunae shape as well as discontinuities in the lacunae volume and LCN connectivity. The bony callus exhibits a 34% higher lacunae number density with 40% larger lacunar volume compared to cortical bone. The presented correlations between LCN architecture and mineral characteristics improves our understanding of how bone develops during healing and may indicate a contribution of osteocytes to bone (re)modeling.

Details

show
hide
Language(s): eng - English
 Dates: 2020-09-112020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.jsb.2020.107616
PMID: 0596
 Degree: -

Event

show

Legal Case

show

Project information

show hide
Project name : Berlin-Brandenburg School for Regenerative Therapies GSC 203
Grant ID : -
Funding program : (272944896)
Funding organization : Deutsche Forschungsgemeinschaft
Project name : Integrated Computational Material, Process and Product Engineering (IC-MPPE)
Grant ID : -
Funding program : COMET (859480)
Funding organization : -

Source 1

show
hide
Title: Journal of Structural Biology
  Abbreviation : J. Struct. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Diego, CA : Elsevier
Pages: - Volume / Issue: 212 (2) Sequence Number: 107616 Start / End Page: - Identifier: ISSN: 1047-8477