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  Fortified coiled coils : enhancing mechanical stability with lactam or metal staples

López-García, P., de Araujo, A. D., Bergues-Pupo, A. E., Tunn, I., Fairlie, D. P., & Blank, K. G. (2020). Fortified coiled coils: enhancing mechanical stability with lactam or metal staples. Angewandte Chemie International Edition. doi:10.1002/anie.202006971.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-0CA0-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-71B1-1
Genre: Journal Article

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 Creators:
López-García, Patricia, Author
de Araujo, Aline D., Author
Bergues-Pupo, Ana E., Author
Tunn, Isabell1, Author              
Fairlie, David P., Author
Blank, Kerstin G.1, Author              
Affiliations:
1Kerstin Blank, Mechano(bio)chemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2301698              

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Free keywords: coiled coil, Peptide stapling, lactam, metal coordination, single-molecule force spectroscopy
 Abstract: Coiled coils (CCs) are powerful supramolecular building blocks for biomimetic materials, increasingly used for their mechanical properties. Here, we introduce helix-inducing macrocyclic constraints, so-called staples, to tune thermodynamic and mechanical stability of CCs. We show that thermodynamic stabilization of CCs against helix uncoiling primarily depends on the number of staples, whereas staple positioning controls CC mechanical stability. Inserting a covalent lactam staple at one key force application point significantly increases the barrier to force-induced CC dissociation and reduces structural deformity. A reversible His-Ni 2+ -His metal staple also increases CC stability, but ruptures upon mechanical loading to allow helix uncoiling. Staple type, position and number are key design parameters in using helical macrocyclic templates for fine-tuning CC properties in emerging biomaterials.

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Language(s): eng - English
 Dates: 2020-09-17
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1002/anie.202006971
DOI: 10.1002/ange.202006971
BibTex Citekey: doi:10.1002/anie.202006971
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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1433-7851

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Title: Angewandte Chemie
  Abbreviation : Angew. Chem.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0044-8249