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  Droplet-based cytotoxicity assay: implementation of time-efficient screening of antitumor activity of natural killer cells

Antona, S., Platzman, I., & Spatz, J. P. (2020). Droplet-based cytotoxicity assay: implementation of time-efficient screening of antitumor activity of natural killer cells. ACS Omega, 5(38), 24674-24683. doi:10.1021/acsomega.0c03264.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-0A65-D Version Permalink: https://hdl.handle.net/21.11116/0000-0007-910B-9
Genre: Journal Article

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 Creators:
Antona, Silvia, Author
Platzman, Ilia1, Author           
Spatz, Joachim P.1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: Liquids, Assays, Cells, Quantum confinement, Toxicity
 Abstract: Natural killer (NK) cells are key players of the innate immune system. Due to their rapid cytotoxicity against infectious pathogens, hematologic malignancies, and solid tumors, NK cells represent solid candidates for cell-based immunotherapy. Despite the progress made in recent years, the heterogeneity in their cytotoxic behavior represents a drawback. With the goal of screening the intrinsic diversity of NK cells, droplet-based microfluidic technology is exploited to develop a single-cell time-efficient cytotoxicity assay. Toward this end, NK-92 cells are coencapsulated with hematological tumor cell lines in water-in-oil droplets of different sizes and their cytotoxic activity is evaluated. The effect of droplet-based confinement on NK cytotoxicity is investigated by controlling the droplet volume. The successful optimization of the droplet size allows for time efficiency compared to cytotoxicity assays based on flow cytometry. Additionally, the ability of individual NK-92 cells to kill multiple target cells in series is explored, expanding the knowledge about the serial killing process dynamics. The developed droplet-based microfluidic assay does not require the labeling of NK cells and represents a step toward developing of a forthcoming process for the selection of NK cells with the highest cytotoxicity against specific targets.

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Language(s): eng - English
 Dates: 2020-07-072020-09-042020-09-172020
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acsomega.0c03264
 Degree: -

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Title: ACS Omega
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 5 (38) Sequence Number: - Start / End Page: 24674 - 24683 Identifier: ISSN: 2470-1343
CoNE: https://pure.mpg.de/cone/journals/resource/2470-1343