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  Crystallization and preliminary X-ray crystallographic analysis of DFPase from Loligo vulgaris

Scharff, E. I., Lücke, C., Fritzsch, G., Koepke, J., Hartleib, J., Dierl, S., et al. (2001). Crystallization and preliminary X-ray crystallographic analysis of DFPase from Loligo vulgaris. Acta Crystallographica Section D-Biological Crystallography, 57(1), 148-149. doi:10.1107/S0907444900014232.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-0B2B-E Version Permalink: https://hdl.handle.net/21.11116/0000-0007-32E5-E
Genre: Journal Article

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 Creators:
Scharff, Eileen I.1, Author
Lücke, Christian1, Author
Fritzsch, Günter2, Author           
Koepke, Jürgen2, Author           
Hartleib, Judith1, Author
Dierl, Stefan1, Author
Rüterjans, Heinz1, Author
Affiliations:
1Institute of Biophysical Chemistry, Johann Wolfgang Goethe-University, 60439 Frankfurt/Main, Germany, ou_persistent22              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: diidopropylfluorophosphatease; organophosphorus triester hydrolysis; phosphotriesterases
 Abstract: 'Squid-type' diisopropylØuorophosphatases (DFPases), a subclass of the phosphotriesterases, are enzymes capable of hydrolysing organophosphorus nerve agents. To date, no three-dimensionalstructure of a `squid-type' DFPase is known. Here, the crystallization of the DFPase originally isolated from head ganglion of the squid Loligo vulgarisis reported. The protein has been heterologously expressed in Escherichia coli, purified to homogeneity and subsequently crystallized. The protein crystals belong to space group P212121, with unit-cell parametersa= 43.1,b= 82.1,c= 86.6 Å and one monomer per asymmetric unit. Under cryoconditions (120 K) the crystals diffracted beyond 2.0 Å using a Cu rotating-anode X-raygenerator

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Language(s): eng - English
 Dates: 2000-07-052000-10-102001-01-01
 Publication Status: Issued
 Pages: 2
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1107/S0907444900014232
 Degree: -

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Title: Acta Crystallographica Section D-Biological Crystallography
Source Genre: Journal
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Publ. Info: [Copenhagen, Denmark : Published for the International Union of Crystallography by Munksgaard]
Pages: - Volume / Issue: 57 (1) Sequence Number: - Start / End Page: 148 - 149 Identifier: ISSN: 0907-4449
CoNE: https://pure.mpg.de/cone/journals/resource/954925562619_1