Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  3D diffractive imaging of nanoparticle ensembles using an x-ray laser

Ayyer, K., Xavier, P. L., Bielecki, J., Shen, Z., Daurer, B. J., Samanta, A. K., et al. (2021). 3D diffractive imaging of nanoparticle ensembles using an x-ray laser. Optica, 8(1), 15-23. doi:10.1364/OPTICA.410851.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
optica-8-1-15.pdf (Verlagsversion), 17MB
Name:
optica-8-1-15.pdf
Beschreibung:
Open Access. - Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.
OA-Status:
Keine Angabe
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
2021
Copyright Info:
© Optical Society of America
:
suppl.zip (Ergänzendes Material), 13MB
Name:
suppl.zip
Beschreibung:
Supplementary Information (pdf); Visualization (mp4): Intensity isosurfaces with varying levels for the three larger samples; Visualization (mp4): Rotating versions of electron density isosurfaces shown in Fig. 5(a).
OA-Status:
Keine Angabe
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/zip / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://arxiv.org/abs/2007.13597 (Preprint)
Beschreibung:
-
OA-Status:
Keine Angabe
externe Referenz:
https://dx.doi.org/10.1364/OPTICA.410851 (Verlagsversion)
Beschreibung:
-
OA-Status:
Keine Angabe

Urheber

einblenden:
ausblenden:
 Urheber:
Ayyer, K.1, 2, 3, Autor           
Xavier, P. L.4, Autor
Bielecki, J.4, Autor
Shen, Z.4, Autor
Daurer, B. J.4, Autor
Samanta, A. K.4, Autor
Awel, S.4, Autor
Bean, R.4, Autor
Barty, A.4, Autor
Ekeberg, T.4, Autor
Estillore, A. D.4, Autor
Fangohr, H.5, 6, Autor           
Giewekemeyer, K.4, Autor
Hunter, M. S.4, Autor
Karnevskiy, M.4, Autor
Kirian, R. A.4, Autor
Kirkwood, H.4, Autor
Kim, Y.4, Autor
Koliyadu, J.4, Autor
Lange, H.4, Autor
Letrun, R.4, AutorLübke, J.4, AutorMichelat, T.4, AutorMorgan, A. J.4, AutorRoth, N.4, AutorSato, T.4, AutorSikorski, M.4, AutorSchulz, F.4, AutorSpence, J. C. H.4, AutorVagovic, P.4, AutorWollweber, T.1, 2, 3, 7, Autor           Worbs, L.4, AutorYefanov, O.4, AutorZhuang, Y.1, 2, Autor           Maia, F. R. N. C.4, AutorHorke, D. A.4, AutorKüpper, J.4, AutorLoh, N. D.4, AutorMancuso, A. P.4, AutorChapman, H. N.4, Autor mehr..
Affiliations:
1Computational Nanoscale Imaging, Condensed Matter Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3012829              
2Center for Free-Electron Laser Science, ou_persistent22              
3The Hamburg Center for Ultrafast Imaging, Universität Hamburg, ou_persistent22              
4external, ou_persistent22              
5European XFEL, ou_persistent22              
6Computational Science, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3267028              
7Department of Physics, Universität Hamburg, ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Single particle imaging at x-ray free electron lasers (XFELs) has the potential to determine the structure and dynamics of single biomolecules at room temperature. Two major hurdles have prevented this potential from being reached, namely, the collection of sufficient high-quality diffraction patterns and robust computational purification to overcome structural heterogeneity. We report the breaking of both of these barriers using gold nanoparticle test samples, recording around 10 million diffraction patterns at the European XFEL and structurally and orientationally sorting the patterns to obtain better than 3-nm-resolution 3D reconstructions for each of four samples. With these new developments, integrating advancements in x-ray sources, fast-framing detectors, efficient sample delivery, and data analysis algorithms, we illuminate the path towards sub-nanometer biomolecular imaging. The methods developed here can also be extended to characterize ensembles that are inherently diverse to obtain their full structural landscape.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2020-11-032020-10-072020-11-202020-12-242021-01
 Publikationsstatus: Erschienen
 Seiten: 9
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: arXiv: 2007.13597
DOI: 10.1364/OPTICA.410851
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden: ausblenden:
Projektname : We thank Rick Millane for helpful discussions. We acknowledge European XFEL in Schenefeld, Germany, for provision of x-ray free-electron laser beamtime at Scientific Instrument SPB/SFX and thank the staff for their assistance. We thank the DESY NanoLab, CSSB cryoEM user facility and the XBI labs at EuXFEL for access to electron microscopy resources and their staff for their help. J.C.H.S. and R.A.K. acknowledge support from the National Science Foundation BioXFEL. J.R.N.C.M. acknowledges support from the Swedish Research Council, Röntgen-Ångström Cluster, and Carl Tryggers Foundation for Scientific Research. P.L.X. acknowledges a fellowship from the Joachim Herz Stiftung. P.L.X. and H.N.C. acknowledge support from the Human Frontiers Science Program.
Grant ID : -
Förderprogramm : -
Förderorganisation : -

Quelle 1

einblenden:
ausblenden:
Titel: Optica
  Kurztitel : Optica
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, DC, United States : The Optical Society
Seiten: - Band / Heft: 8 (1) Artikelnummer: - Start- / Endseite: 15 - 23 Identifikator: ISSN: 2334-2536
CoNE: https://pure.mpg.de/cone/journals/resource/2334-2536