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  3D diffractive imaging of nanoparticle ensembles using an x-ray laser

Ayyer, K., Xavier, P. L., Bielecki, J., Shen, Z., Daurer, B. J., Samanta, A. K., et al. (2021). 3D diffractive imaging of nanoparticle ensembles using an x-ray laser. Optica, 8(1), 15-23. doi:10.1364/OPTICA.410851.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0007-0C93-6 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-C62B-6
Genre: Journal Article

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Supplementary Information (pdf); Visualization (mp4): Intensity isosurfaces with varying levels for the three larger samples; Visualization (mp4): Rotating versions of electron density isosurfaces shown in Fig. 5(a).
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https://arxiv.org/abs/2007.13597 (Preprint)
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 Creators:
Ayyer, K.1, 2, 3, Author           
Xavier, P. L.4, Author
Bielecki, J.4, Author
Shen, Z.4, Author
Daurer, B. J.4, Author
Samanta, A. K.4, Author
Awel, S.4, Author
Bean, R.4, Author
Barty, A.4, Author
Ekeberg, T.4, Author
Estillore, A. D.4, Author
Fangohr, H.5, 6, Author           
Giewekemeyer, K.4, Author
Hunter, M. S.4, Author
Karnevskiy, M.4, Author
Kirian, R. A.4, Author
Kirkwood, H.4, Author
Kim, Y.4, Author
Koliyadu, J.4, Author
Lange, H.4, Author
Letrun, R.4, AuthorLübke, J.4, AuthorMichelat, T.4, AuthorMorgan, A. J.4, AuthorRoth, N.4, AuthorSato, T.4, AuthorSikorski, M.4, AuthorSchulz, F.4, AuthorSpence, J. C. H.4, AuthorVagovic, P.4, AuthorWollweber, T.1, 2, 3, 7, Author           Worbs, L.4, AuthorYefanov, O.4, AuthorZhuang, Y.1, 2, Author           Maia, F. R. N. C.4, AuthorHorke, D. A.4, AuthorKüpper, J.4, AuthorLoh, N. D.4, AuthorMancuso, A. P.4, AuthorChapman, H. N.4, Author more..
Affiliations:
1Computational Nanoscale Imaging, Condensed Matter Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3012829              
2Center for Free-Electron Laser Science, ou_persistent22              
3The Hamburg Center for Ultrafast Imaging, Universität Hamburg, ou_persistent22              
4external, ou_persistent22              
5European XFEL, ou_persistent22              
6Computational Science, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_3267028              
7Department of Physics, Universität Hamburg, ou_persistent22              

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 Abstract: Single particle imaging at x-ray free electron lasers (XFELs) has the potential to determine the structure and dynamics of single biomolecules at room temperature. Two major hurdles have prevented this potential from being reached, namely, the collection of sufficient high-quality diffraction patterns and robust computational purification to overcome structural heterogeneity. We report the breaking of both of these barriers using gold nanoparticle test samples, recording around 10 million diffraction patterns at the European XFEL and structurally and orientationally sorting the patterns to obtain better than 3-nm-resolution 3D reconstructions for each of four samples. With these new developments, integrating advancements in x-ray sources, fast-framing detectors, efficient sample delivery, and data analysis algorithms, we illuminate the path towards sub-nanometer biomolecular imaging. The methods developed here can also be extended to characterize ensembles that are inherently diverse to obtain their full structural landscape.

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Language(s): eng - English
 Dates: 2020-11-032020-10-072020-11-202020-12-242021-01
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: arXiv: 2007.13597
DOI: 10.1364/OPTICA.410851
 Degree: -

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Project name : We thank Rick Millane for helpful discussions. We acknowledge European XFEL in Schenefeld, Germany, for provision of x-ray free-electron laser beamtime at Scientific Instrument SPB/SFX and thank the staff for their assistance. We thank the DESY NanoLab, CSSB cryoEM user facility and the XBI labs at EuXFEL for access to electron microscopy resources and their staff for their help. J.C.H.S. and R.A.K. acknowledge support from the National Science Foundation BioXFEL. J.R.N.C.M. acknowledges support from the Swedish Research Council, Röntgen-Ångström Cluster, and Carl Tryggers Foundation for Scientific Research. P.L.X. acknowledges a fellowship from the Joachim Herz Stiftung. P.L.X. and H.N.C. acknowledge support from the Human Frontiers Science Program.
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Title: Optica
  Abbreviation : Optica
Source Genre: Journal
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Publ. Info: Washington, DC, United States : The Optical Society
Pages: - Volume / Issue: 8 (1) Sequence Number: - Start / End Page: 15 - 23 Identifier: ISSN: 2334-2536
CoNE: https://pure.mpg.de/cone/journals/resource/2334-2536