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  Cryoelectron Tomography Reveals Nanoscale Organization of the Cytoskeleton and Its Relation to Microtubule Curvature Inside Cells

Chakraborty, S., Mahamid, J., & Baumeister, W. (2020). Cryoelectron Tomography Reveals Nanoscale Organization of the Cytoskeleton and Its Relation to Microtubule Curvature Inside Cells. Structure, 28(9), 991-1003.e4. doi:10.1016/j.str.2020.05.013.

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 Creators:
Chakraborty, Saikat1, Author              
Mahamid, Julia1, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: INTERMEDIATE-FILAMENTS; DYNAMIC INSTABILITY; MOLECULAR SOCIOLOGY; ELECTRON TOMOGRAMS; FLEXURAL RIGIDITY; ACTIN-FILAMENTS; MECHANICS; BREAKAGE; LATTICE; POLYMERIZATIONBiochemistry & Molecular Biology; Biophysics; Cell Biology;
 Abstract: Microtubules (MTs) are the most rigid elements of the cytoskeleton with in vitro persistence lengths (L-p) in the range of 1-6 mm. In cellular environments, however, MTs often appear strongly curved. This has been attributed to the forces acting upon them in situ where they are embedded in composite networks of different cytoskeletal elements. Hitherto, the nanoscale organization of these networks has remained largely uncharacterized. Cryo-electron tomography (cryo-ET) allowed to visualize and analyze the in situ structure of cytoskeletal networks in pristinely preserved cellular environments and at high resolution. Here, we studied the molecular organization of MTs and their interactions with the composite cytoskeleton in frozen-hydrated HeLa and P19 cells at different cell-cycle stages. We describe modulation of MT curvature correlated with the surrounding molecular architecture, and show that nanoscale defects occur in curved MTs. The data presented here contribute to constructing realistic models of cytoskeletal biomechanics.

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Language(s): eng - English
 Dates: 2020-062020
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000564932600003
DOI: 10.1016/j.str.2020.05.013
 Degree: -

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Title: Structure
  Other : Structure
Source Genre: Journal
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Publ. Info: London : Cell Press
Pages: - Volume / Issue: 28 (9) Sequence Number: - Start / End Page: 991 - 1003.e4 Identifier: ISSN: 0969-2126
CoNE: https://pure.mpg.de/cone/journals/resource/954927002244_1