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Free keywords:
BRANCHED ACTIN-FILAMENTS; ALPHA-BETA-TUBULIN; INTERMEDIATE-FILAMENTS;
ELECTRON-MICROSCOPY; ARP2/3 COMPLEX; MOLECULAR ARCHITECTURE;
LISTERIA-MONOCYTOGENES; PROTOFILAMENT NUMBERS; DYNAMIC INSTABILITY;
EUKARYOTIC CELLSBiochemistry & Molecular Biology; actin filaments; cryo-electron tomography; in situ architecture;
intermediate filaments; microtubules;
Abstract:
Traditionally, structures of cytoskeletal components have been studied ex situ, that is, with biochemically purified materials. There are compelling reasons to develop approaches to study them in situ in their native functional context. In recent years, cryo-electron tomography emerged as a powerful method for visualizing the molecular organization of unperturbed cellular landscapes with the potential to attain near-atomic resolution. Here, we review recent works on the cytoskeleton using cryo-electron tomography, demonstrating the power of in situ studies. We also highlight the potential of this method in addressing important questions pertinent to the field of cytoskeletal biomechanics.
