English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Single particle imaging with FEL using photon correlations

von Ardenne, B., & Grubmüller, H. (2020). Single particle imaging with FEL using photon correlations. In T. Salditt, A. Egner, & D. Luke (Eds.), Nanoscale Photonic Imaging (pp. 435-455). Cham: Springer. doi:10.1007/978-3-030-34413-9_16.

Item is

Files

show Files
hide Files
:
3254943.pdf (Publisher version), 864KB
Name:
3254943.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
von Ardenne, B.1, Author              
Grubmüller, H.1, Author              
Affiliations:
1Department of Theoretical and Computational Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578631              

Content

show
hide
Free keywords: Single particle imaging; Femtosecond X-ray pulses; Free electron laser; Photon correlations
 Abstract: Scattering experiments with femtosecond high-intensity free-electron laser pulses provide a new route to macromolecular structure determination without the need for crystallization at low material usage. In these experiments, the X-ray pulses are scattered with high repetition on a stream of identical single biomolecules and the scattered photons are recorded on a pixelized detector. The main challenges are the unknown random orientation of the molecule in each shot and the extremely low signal to noise ratio due to the very low expected photon count per scattering image, typically well below the number of over 100 photons required by available analysis methods. The latter currently limits the scattering experiments to nano-crystals or larger virus particles, but the ultimate goal remains to retrieve the atomic structure of single biomolecules. Here, we use photon correlations to overcome the issue with low photon counts and present an approach that can determine the molecular structure de novo from as few as three coherently scattered photons per image. We further validate the method with a small protein (46 residues), show that near-atomic resolution of 3.3 Å is within experimental reach and demonstrate structure determination in the presence of isotropic noise from various sources, indicating that the number of disordered solvent molecules attached to the macromolecular surface should be kept at a minimum. Our correlation method allows to infer structure from images containing multiple particles, potentially opening the method to other types of experiments such as fluctuation X-ray scattering (FXS).

Details

show
hide
Language(s): eng - English
 Dates: 2020-06-102020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-3-030-34413-9_16
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nanoscale Photonic Imaging
Source Genre: Book
 Creator(s):
Salditt, T., Editor
Egner, A., Editor
Luke, D., Editor
Affiliations:
-
Publ. Info: Cham : Springer
Pages: XXII, 634 Volume / Issue: - Sequence Number: - Start / End Page: 435 - 455 Identifier: ISBN: 978-3-030-34412-2

Source 2

show
hide
Title: Topics in Applied Physics
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 134 Sequence Number: - Start / End Page: - Identifier: -