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  From the field to the laboratory: Controlling DNA contamination in human ancient DNA research in the high-throughput sequencing era

Llamas, B., Valverde, G., Fehren-Schmitz, L., Weyrich, L. S., Cooper, A., & Haak, W. (2017). From the field to the laboratory: Controlling DNA contamination in human ancient DNA research in the high-throughput sequencing era. Science and Technology of Archaeological Research: STAR, 3(1), 1-14. doi:/10.1080/20548923.2016.1258824.

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Llamas_From_STAR_2017.pdf (Publisher version), 2MB
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 Creators:
Llamas, Bastien, Author
Valverde, Guido, Author
Fehren-Schmitz, Lars, Author
Weyrich, Laura S, Author
Cooper, Alan, Author
Haak, Wolfgang1, 2, Author                 
Affiliations:
1PALEoRIDER, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2541702              
2Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              

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Free keywords: ancient DNA, contaminating DNA, archaeological sampling
 Abstract: High-Throughput DNA Sequencing (HTS) technologies have changed the way in which we detect and assess DNA contamination in ancient DNA studies. Researchers use computational methods to mine the large quantity of sequencing data to detect characteristic patterns of DNA damage, and to evaluate the authenticity of the results. We argue that unless computational methods can confidently separate authentic ancient DNA sequences from contaminating DNA that displays damage patterns under independent decay processes, prevention and control of DNA contamination should remain a central and critical aspect of ancient human DNA studies. Ideally, DNA contamination can be prevented early on by following minimal guidelines during excavation, sample collection and/or subsequent handling. Contaminating DNA should also be monitored or minimised in the ancient DNA laboratory using specialised facilities and strict experimental procedures. In this paper, we update recommendations to control for DNA contamination from the field to the laboratory, in an attempt to facilitate communication between field archaeologists, anthropologists and ancient DNA researchers. We also provide updated criteria of ancient DNA authenticity for HTS-based studies. We are confident that the procedures outlined here will increase the retrieval of higher proportions of authentic genetic information from valuable archaeological human remains in the future.

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Language(s): eng - English
 Dates: 2017
 Publication Status: Issued
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: /10.1080/20548923.2016.1258824
Other: shh2331
 Degree: -

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Title: Science and Technology of Archaeological Research : STAR
  Other : STAR: Science and Technology of Archaeological Research
Source Genre: Journal
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Publ. Info: Abingdon : Taylor & Francis
Pages: - Volume / Issue: 3 (1) Sequence Number: - Start / End Page: 1 - 14 Identifier: ISSN: 2054-8923
CoNE: https://pure.mpg.de/cone/journals/resource/2054-8923