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  Live-cell super-resolved PAINT imaging of piconewton cellular traction forces

Brockman, J. M., Su, H., Blanchard, A. T., Duan, Y., Meyer, T., Quach, M. E., et al. (2020). Live-cell super-resolved PAINT imaging of piconewton cellular traction forces. Nature Methods, 17, 1018-1024. doi:10.1038/s41592-020-0929-2.

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 Creators:
Brockman, Joshua M.1, Author
Su, Hanquan1, Author
Blanchard, Aaron T.1, Author
Duan, Yuxin1, Author
Meyer, Travis1, Author
Quach, M. Edward1, Author
Glazier, Roxanne1, Author
Bazrafshan, Alisina1, Author
Bender, Rachel L.1, Author
Kellner, Anna V.1, Author
Ogasawara, Hiroaki1, Author
Ma, Rong1, Author
Schueder, Florian2, Author              
Petrich, Brian G.1, Author
Jungmann, Ralf2, Author              
Li, Renhao1, Author
Mattheyses, Alexa L.1, Author
Ke, Yonggang1, Author
Salaita, Khalid1, Author
Affiliations:
1external, ou_persistent22              
2Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              

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Free keywords: SUPERRESOLUTION MICROSCOPY; FOCAL ADHESIONS; 3D ORIENTATION; INTEGRIN; TENSION; ARCHITECTURE; STIFFNESS; GUIDEBiochemistry & Molecular Biology;
 Abstract: Despite the vital role of mechanical forces in biology, it still remains a challenge to image cellular force with sub-100-nm resolution. Here, we present tension points accumulation for imaging in nanoscale topography (tPAINT), integrating molecular tension probes with the DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) technique to map piconewton mechanical events with similar to 25-nm resolution. To perform live-cell dynamic tension imaging, we engineered reversible probes with a cryptic docking site revealed only when the probe experiences forces exceeding a defined mechanical threshold (similar to 7-21 pN). Additionally, we report a second type of irreversible tPAINT probe that exposes its cryptic docking site permanently and thus integrates force history over time, offering improved spatial resolution in exchange for temporal dynamics. We applied both types of tPAINT probes to map integrin receptor forces in live human platelets and mouse embryonic fibroblasts. Importantly, tPAINT revealed a link between platelet forces at the leading edge of cells and the dynamic actin-rich ring nucleated by the Arp2/3 complex.

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Language(s): eng - English
 Dates: 2020-092020-10
 Publication Status: Published in print
 Pages: 27
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000569908500003
DOI: 10.1038/s41592-020-0929-2
 Degree: -

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Title: Nature Methods
  Other : Nature Methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 17 Sequence Number: - Start / End Page: 1018 - 1024 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556