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  Dual Functions of a Rubisco Activase in Metabolic Repair and Recruitment to Carboxysomes.

Flecken, M., Wang, H., Popilka, L., Hartl, F. U., Bracher, A., & Hayer-Hartl, M. (2020). Dual Functions of a Rubisco Activase in Metabolic Repair and Recruitment to Carboxysomes. Cell, 183(2), 457-473.e20. doi:10.1016/j.cell.2020.09.010.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-8257-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-8258-3
Genre: Journal Article

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 Creators:
Flecken, Mirkko1, Author              
Wang, Huping1, Author              
Popilka, Leonhard1, Author              
Hartl, F. Ulrich1, Author              
Bracher, Andreas1, Author              
Hayer-Hartl, Manajit1, Author              
Affiliations:
1Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565152              

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Free keywords: Nicotiana tabacum; Nostoc sp. PCC 7120; Rubisco; Rubisco activase; X-ray crystallography; carboxysome; cryo-electron microscopy; cyanobacteria; liquid-liquid phase separation; metabolic repair
 Abstract: Rubisco, the key enzyme of CO2 fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis of cyanobacterial Rca, a close homolog of plant Rca. In the Rca:Rubisco complex, Rca is positioned over the Rubisco catalytic site under repair and pulls the N-terminal tail of the large Rubisco subunit (RbcL) into the hexamer pore. Simultaneous displacement of the C terminus of the adjacent RbcL opens the catalytic site for inhibitor release. An alternative interaction of Rca with Rubisco is mediated by C-terminal domains that resemble the small Rubisco subunit. These domains, together with the N-terminal AAA+ hexamer, ensure that Rca is packaged with Rubisco into carboxysomes. The cyanobacterial Rca is a dual-purpose protein with functions in Rubisco repair and carboxysome organization. Copyright © 2020 Elsevier Inc. All rights reserved.

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Language(s): eng - English
 Dates: 2020-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 32979320
DOI: 10.1016/j.cell.2020.09.010
 Degree: -

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Title: Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 183 (2) Sequence Number: - Start / End Page: 457 - 473.e20 Identifier: ISSN: 0092-8674
CoNE: https://pure.mpg.de/cone/journals/resource/954925463183