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  Rabgap1 promotes recycling of active beta1 integrins to support effective cell migration.

Samarelli, A. V., Ziegler, T., Meves, A., Fässler, R., & Böttcher, R. T. (2020). Rabgap1 promotes recycling of active beta1 integrins to support effective cell migration. Journal of Cell Science, 133(18): jcs243683. doi:10.1242/jcs.243683.

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Genre: Journal Article

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 Creators:
Samarelli, Anna V.1, Author              
Ziegler, Tilman1, Author              
Meves, Alexander1, Author              
Fässler, Reinhard1, Author              
Böttcher, Ralph T.1, Author              
Affiliations:
1Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

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Free keywords: Active beta1 integrins; Cancer cell invasion; Cell migration; Integrin recycling; Rabgap1
 Abstract: Integrin function depends on the continuous internalization of integrins and their subsequent endosomal recycling to the plasma membrane to drive adhesion dynamics, cell migration and invasion. Here we assign a pivotal role for Rabgap1 (GAPCenA) in the recycling of endocytosed active beta1 integrins to the plasma membrane. The phosphotyrosine-binding (PTB) domain of Rabgap1 binds to the membrane-proximal NPxY motif in the cytoplasmic domain of beta1 integrin subunits on endosomes. Silencing Rabgap1 in mouse fibroblasts leads to the intracellular accumulation of active beta1 integrins, alters focal adhesion formation, and decreases cell migration and cancer cell invasion. Functionally, Rabgap1 facilitates active beta1 integrin recycling to the plasma membrane through attenuation of Rab11 activity. Taken together, our results identify Rabgap1 as an important factor for conformation-specific integrin trafficking and define the role of Rabgap1 in beta1-integrin-mediated cell migration in mouse fibroblasts and breast cancer cells. © 2020. Published by The Company of Biologists Ltd.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 32843574
DOI: 10.1242/jcs.243683
 Degree: -

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Project name : SFB914, project A05 to R.T.B. and R.F.
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Funding program : -
Funding organization : Deutsche Forschungsgemeinschaft
Project name : K08 CA215105 to A.M.
Grant ID : -
Funding program : -
Funding organization : National Institutes of Health

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Title: Journal of Cell Science
Source Genre: Journal
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Publ. Info: Cambridge, U.K. : Co. of Biologists
Pages: - Volume / Issue: 133 (18) Sequence Number: jcs243683 Start / End Page: - Identifier: ISSN: 0021-9533
CoNE: https://pure.mpg.de/cone/journals/resource/954925326678