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  Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.

Klena, N., Le Guennec, M., Tassin, A.-M., Van den Hoek, H., Erdmann, P. S., Schaffer, M., et al. (2020). Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography. The EMBO Journal, 39(22): e106246. doi:10.15252/embj.2020106246.

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© 2020 The Authors. Published under the terms of the CC BY 4.0 license

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 Urheber:
Klena, Nikolai1, Autor
Le Guennec, Maeva1, Autor
Tassin, Anne-Marie1, Autor
Van den Hoek, Hugo2, Autor           
Erdmann, Philipp S.2, Autor           
Schaffer, Miroslava2, Autor           
Geimer, Stefan1, Autor
Aeschlimann, Gabriel1, Autor
Kovacik, Lubomir1, Autor
Sadian, Yashar1, Autor
Goldie, Kenneth N1, Autor
Stahlberg, Henning1, Autor
Engel, Benjamin D.2, Autor           
Hamel, Virginie1, Autor
Guichard, Paul1, Autor
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Schlagwörter: insitu; cartwheel; centriole; cryo-electron tomography; cryo-focused ion beam milling
 Zusammenfassung: Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture and evolutionary conservation remain relatively unexplored. Here, using cryo-electron tomography of centrioles from four evolutionarily distant species, we report on the architectural diversity of the centriole's proximal cartwheel-bearing region. Our work reveals that the cartwheel central hub is constructed from a stack of paired rings with cartwheel inner densities inside. In both Paramecium and Chlamydomonas, the repeating structural unit of the cartwheel has a periodicity of 25nm and consists of three ring pairs, with 6 radial spokes emanating and merging into a single bundle that connects to the microtubule triplet via the D2-rod and the pinhead. Finally, we identified that the cartwheel is indirectly connected to the A-C linker through the triplet base structure extending from the pinhead. Together, our work provides unprecedented evolutionary insights into the architecture of the centriole proximal region, which underlies centriole biogenesis. © 2020 The Authors. Published under the terms of the CC BY 4.0 license.

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Sprache(n): eng - English
 Datum: 2020
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 32954513
DOI: 10.15252/embj.2020106246
 Art des Abschluß: -

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Titel: The EMBO Journal
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Nature Publishing Group
Seiten: - Band / Heft: 39 (22) Artikelnummer: e106246 Start- / Endseite: - Identifikator: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061_1