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  Peptide-PAINT Super-Resolution Imaging Using Transient Coiled Coil Interactions.

Eklund, A. S., Ganji, M., Gavins, G., Seitz, O., & Jungmann, R. (2020). Peptide-PAINT Super-Resolution Imaging Using Transient Coiled Coil Interactions. Nano Letters, 20(9), 6732-6737. doi:10.1021/acs.nanolett.0c02620.

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 Creators:
Eklund, Alexandra S.1, Author              
Ganji, Mahipal1, Author              
Gavins, Georgina2, Author
Seitz, Oliver2, Author
Jungmann, Ralf1, Author              
Affiliations:
1Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society, ou_2149679              
2external, ou_persistent22              

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Free keywords: DNA-PAINT; Peptide-PAINT; Super-resolution; coiled coil interactions; single-molecule imaging; transient binding
 Abstract: Super-resolution microscopy is transforming research in the life sciences by enabling the visualization of structures and interactions on the nanoscale. DNA-PAINT is a relatively easy-to-implement single-molecule-based technique, which uses the programmable and transient interaction of dye-labeled oligonucleotides with their complements for super-resolution imaging. However, similar to many imaging approaches, it is still hampered by the subpar performance of labeling probes in terms of their large size and limited labeling efficiency. To overcome this, we here translate the programmability and transient binding nature of DNA-PAINT to coiled coil interactions of short peptides and introduce Peptide-PAINT. We benchmark and optimize its binding kinetics in a single-molecule assay and demonstrate its super-resolution capability using self-assembled DNA origami structures. Peptide-PAINT outperforms classical DNA-PAINT in terms of imaging speed and efficiency. Finally, we prove the suitability of Peptide-PAINT for cellular super-resolution imaging by visualizing the microtubule and vimentin network in fixed cells.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: We thank Stefan Pettera and Stephan Uebel from the MPIB peptide synthesis service for the production of the peptides and for their advice regarding the peptide modifications.
 Rev. Type: -
 Identifiers: ISI: 32787168
DOI: 10.1021/acs.nanolett.0c02620
 Degree: -

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Project name : Marie Skłodowska-Curie grant agreement no. 796606
Grant ID : 796606
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : Emmy Noether Program (DFG JU 2957/1-1), the SFB1032 (project A11)
Grant ID : -
Funding program : -
Funding organization : German Research Foundation
Project name : ERC Starting Grant (MolMap, grant agreement number 680241)
Grant ID : 680241
Funding program : -
Funding organization : European Research Council

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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 20 (9) Sequence Number: - Start / End Page: 6732 - 6737 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403