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  Non-specific activation of the epithelial sodium channel by the CFTR chloride channel

Nagel, G., Szellas, T., Riordan, J. R., Friedrich, T., & Hartung, K. (2001). Non-specific activation of the epithelial sodium channel by the CFTR chloride channel. EMBO Reports, 2(3), 249-254. doi:10.1093/embo-reports/kve045.

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 Creators:
Nagel, Georg1, Author           
Szellas, Tanjef1, Author           
Riordan, John R.2, Author
Friedrich, Thomas1, Author           
Hartung, Klaus1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2S.C. Johnson Medical Research Center, Mayo Clinic, 13400 E Shea Blvd, Scottsdale, AZ‐85259 USA, ou_persistent22              

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 Abstract: The genetic disease cystic fibrosis is caused by mutation of the gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR). Controversial studies reported regulation of the epithelial sodium channel (ENaC) by CFTR. We found that uptake of 22Na+ through ENaC is modulated by activation of CFTR in oocytes, coexpressing CFTR and ENaC, depending on extracellular chloride concentration. Furthermore we found that the effect of CFTR activation could be mimicked by other chloride channels. Voltage- and patch-clamp measurements, however, showed neither stimulation nor inhibition of ENaC-mediated conductance by activated CFTR. We conclude that the observed modulation of 22Na+ uptake by activated CFTR is due to the effect of CFTR-mediated chloride conductance on the membrane potential. These findings argue against the notion of a specific influence of CFTR on ENaC and emphasize the chloride channel function of CFTR.

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Language(s): eng - English
 Dates: 2001-01-122000-11-212001-01-192001-03-01
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1093/embo-reports/kve045
PMID: 11266369
 Degree: -

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Title: EMBO Reports
  Other : EMBO Rep.
Source Genre: Journal
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Publ. Info: Oxford, UK : Published for EMBO by Oxford University Press
Pages: - Volume / Issue: 2 (3) Sequence Number: - Start / End Page: 249 - 254 Identifier: ISSN: 1469-221X
CoNE: https://pure.mpg.de/cone/journals/resource/110978984569661