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  The anion conductance of the glutamate transporter EAAC1 depends on the direction of glutamate transport

Watzke, N., & Grewer, C. (2001). The anion conductance of the glutamate transporter EAAC1 depends on the direction of glutamate transport. FEBS Letters, 503(2-3), 121-125. doi:10.1016/s0014-5793(01)02715-6.

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 Creators:
Watzke, Natalie1, Author           
Grewer, Christof1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

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Free keywords: Glutamate transporter; Reverse transport; dl-threo-β-Benzyloxyaspartate; Rapid kinetics
 Abstract: The steady-state and pre-steady-state kinetics of glutamate transport by the neuronal glutamate transporter EAAC1 were determined under conditions of outward glutamate transport and compared to those found for the inward transport mode. In both transport modes, the glutamate-induced current is composed of two components, the coupled transport current and the uncoupled anion current, and inhibited by a specific non-transportable inhibitor. Furthermore, the glutamate-independent leak current is observed in both transport modes. Upon a glutamate concentration jump outward transport currents show a distinct transient phase that deactivates within 15 ms. The results demonstrate that the general properties of EAAC1 are symmetric, but the rates of substrate transport and anion flux are asymmetric with respect to the orientation of the substrate binding site in the membrane. Therefore, the EAAC1 anion conductance differs from normal ligand-gated ion channels in that it can be activated by glutamate and Na+ from both sides of the membrane.

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Language(s): eng - English
 Dates: 2001-05-312001-07-102001-07-262001-08-17
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/s0014-5793(01)02715-6
PMID: 11513867
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 503 (2-3) Sequence Number: - Start / End Page: 121 - 125 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501