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  Monoclonal antibodies against different epitopes of peptide hormones

Jurzak, M., Boer, R., Fritzsch, G., Kojro, E., & Fahrenholz, F. (1990). Monoclonal antibodies against different epitopes of peptide hormones. European Journal of Biochemistry, 190(1), 45-52. doi:10.1111/j.1432-1033.1990.tb15543.x.

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Genre: Journal Article

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 Creators:
Jurzak, Mirek1, Author              
Boer, Rainer1, Author              
Fritzsch, Günter2, Author              
Kojro, Elzbieta1, Author              
Fahrenholz, Falk1, Author              
Affiliations:
1Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_3273414              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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 Abstract: In order to produce monoclonal antibodies directed against different epitopes of the neurohypophyseal hormone vasopressin, the hormone was coupled to carrier proteins via photoreactive groups at different positions in the vasopressin sequence: [2‐(4‐azidophenylalanine), 8‐arginine]vasopressin (peptide P1, photoreactive group at position 2) and desamino‐[8‐N6‐(4‐azidophenylamidino)lysine]vasopressin (peptide P2, photoreactive group at position 8) were conjugated to thyroglobulin by flash photolysis. Monoclonal antibodies against these conjugates bound {[3H]8‐arginine}vasopressin with dissociation constants ranging over 40–400 nM. Epitope analysis by means of competitive ELISA showed that the monoclonal antibody obtained with peptide P1 as hapten was directed against the C‐terminal acyclic tripeptide when its conformation was stabilized by interaction with the disulphide‐linked cyclic hexapeptide. In contrast, the epitope analysis of three monoclonal anti‐(peptide P2) antibodies demonstrated that they recognized antigenic determinants in the cyclic hexapeptide ring, mainly the hydrophobic surface formed by Tyr2 and Phe3. Our results suggest that monoclonal antibodies against different epitopes in small peptide hormones can be generated selectively by using photoreactive peptides in such a way that different antigenic sites are exposed in the hapten‐carrier conjugate.

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Language(s): eng - English
 Dates: 1989-10-181990-01-262005-03-031990-05
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1111/j.1432-1033.1990.tb15543.x
PMID: 1694759
 Degree: -

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Title: European Journal of Biochemistry
Source Genre: Journal
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Publ. Info: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Pages: - Volume / Issue: 190 (1) Sequence Number: - Start / End Page: 45 - 52 Identifier: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040