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  Application of STED imaging for chromatin studies

Kostiuk, G., Bucevicius, J., Gerasimaite, R., & Lukinavicius, G. (2019). Application of STED imaging for chromatin studies. Journal of Physics D, 52(50): 504003. doi:10.1088/1361-6463/ab4410.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-2C52-C Version Permalink: http://hdl.handle.net/21.11116/0000-0007-2C56-8
Genre: Journal Article

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Kostiuk, G.1, Author              
Bucevicius, J.1, Author              
Gerasimaite, R.1, Author              
Lukinavicius, G.1, Author              
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              

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 Abstract: Chromatin is the information center of a cell. It comprises proteins and nucleic acids that form a highly complex and dynamic structure within the nucleus. Its multiple organization levels span from micrometre to nanometre scale. For many years, the lower levels of chromatin organization have been beyond the resolution limit of fluorescent microscopy, thus impeding research on nucleus architecture, transcription, translation and DNA repair. Recent development in super-resolution fluorescence microscopy enables us to more easily observe objects at the nanometre scale and allows the study of complex cellular structures at unprecedented detail. This review focuses on the application of stimulated emission depletion microscopy for imaging two main components of the chromatin-DNA and the proteins interacting with it.

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Language(s): eng - English
 Dates: 2019-09-302019-12-11
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1088/1361-6463/ab4410
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Title: Journal of Physics D
Source Genre: Journal
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Pages: 11 Volume / Issue: 52 (50) Sequence Number: 504003 Start / End Page: - Identifier: -