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  The Structure of the V1-ATPase Determined by Three-Dimensional Electron Microscopy of Single Particles

Radermacher, M., Ruiz, T., Wieczorek, H., & Grüber, G. (2001). The Structure of the V1-ATPase Determined by Three-Dimensional Electron Microscopy of Single Particles. Journal of Structural Biology, 135(1), 26-37. doi:10.1006/jsbi.2001.4395.

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 Creators:
Radermacher, Michael1, Author           
Ruiz, Teresa1, Author           
Wieczorek, Helmut2, Author
Grüber, Gerhard3, Author
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
2Fachbereich Biologie/Chemie, Abteilung Tierphysiologie, Universität Osnabrück, 49069, Osnabrück, Germany, ou_persistent22              
3Universität des Saarlandes, Fachrichtung 2.5-Biophysik, Universitätsbau 76, 66421, Homburg, Germany, ou_persistent22              

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Free keywords: H+V-ATPase; vacuolar-type ATPase; Manduca sexta; three-dimensional reconstruction; electron microscopy; simultaneous alignment; Radon transforms CTF correction
 Abstract: We determined the structure of the V1-ATPase from Manduca sexta to a resolution of 1.8 nm, which for the first time reveals internal features of the enzyme. The V1-ATPase consists of a headpiece of 13.5 nm in diameter, with six elongated subunits, A3 and B3, of approximately equal size, and a stalk of 6 nm in length that connects V1 with the membrane-bound domain, V0. At the center of the molecule is a cavity that extends throughout the length of the A3B3 hexamer. Inside the cavity the central stalk can be seen connected to only two of the catalytic A subunits. The structure was obtained by a combination of the Random Conical Reconstruction Technique and angular refinements. Additional recently developed techniques that were used include methods for simultaneous translational rotational alignment of the 0° images, contrast transfer function correction for tilt images, and the Two-Step Radon Inversion Algorithm.

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Language(s): eng - English
 Dates: 2001-06-142001-02-052002-05-252001-07-01
 Publication Status: Issued
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1006/jsbi.2001.4395
 Degree: -

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Title: Journal of Structural Biology
  Abbreviation : J. Struct. Biol.
Source Genre: Journal
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Publ. Info: San Diego, CA : Elsevier
Pages: - Volume / Issue: 135 (1) Sequence Number: - Start / End Page: 26 - 37 Identifier: ISSN: 1047-8477
CoNE: https://pure.mpg.de/cone/journals/resource/954922650160