English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Grade C molar-incisor pattern periodontitis subgingival microbial profile before and after treatment

Velsko, I. M., Harrison, P., Chalmers, N., Barb, J., Huang, H., Aukhil, I., et al. (2020). Grade C molar-incisor pattern periodontitis subgingival microbial profile before and after treatment. Journal of oral microbiology, 12(1): 1814674, pp. 1-13. doi:10.1080/20002297.2020.1814674.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0007-3356-F Version Permalink: http://hdl.handle.net/21.11116/0000-0007-3357-E
Genre: Journal Article

Files

show Files
hide Files
:
shh2714.pdf (Publisher version), 3MB
Name:
shh2714.pdf
Description:
OA
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Velsko, Irina Marie1, Author              
Harrison, Peter, Author
Chalmers, Natalia, Author
Barb, Jennifer, Author
Huang, Hong, Author
Aukhil, Ikramuddin, Author
Shaddox, Luciana, Author
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              

Content

show
hide
Free keywords: Aggressive periodontitis, subgingival microbiota, A.a., therapy, microarray
 Abstract: AIM: This study evaluated the influence of periodontal therapy on the microbiological profile of individuals with Grade C Molar-Incisor Pattern Periodontitis (C/MIP). METHODS: Fifty-three African-American participants between the ages of 5–25, diagnosed with C/MIP were included. Patients underwent full mouth mechanical debridement with systemic antibiotics (metronidazole 250 mg + amoxicillin 500 mg, tid, 7 days). Subgingival samples were collected from a diseased and a healthy site from each individual prior to treatment and at 3, 6, 12, 18 and 24 months after therapy from the same sites. Samples were subjected to a 16S rRNA gene based-microarray. RESULTS: Treatment was effective in reducing the main clinical parameters of disease. Aggregatibacter actinomycetemcomitans (A.a.) was the strongest species associated with diseased sites. Other species associated with diseased sites were Treponema lecithinolyticum and Tannerella forsythia. Species associated with healthy sites were Rothia dentocariosa/mucilaginosa, Eubacterium yurii, Parvimonas micra, Veillonella spp., Selenomonas spp., and Streptococcus spp. Overall, treatment was effective in strongly reducing A.a. and other key pathogens, as well as increasing health-associated species. These changes were maintained for at least 6 months. CONCLUSIONS:Treatment reduced putative disease-associated species, particularly A.a., and shifted the microbial profile to more closely resemble a healthy-site profile. (Clinicaltrials.gov registration #NCT01330719).

Details

show
hide
Language(s): eng - English
 Dates: 2020-09-13
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: Introduction

Methods
- Demographics of the study population
- Clinical measurements
- Collection of bacterial subgingival biofilm
- Periodontal therapy
- DNA isolation and microarray analysis
- Statistical analyses

Results

Discussion
 Rev. Type: Peer
 Identifiers: DOI: 10.1080/20002297.2020.1814674
Other: shh2714
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of oral microbiology
  Abbreviation : J Oral Microbiol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bålsta : Co-Action Publishing
Pages: - Volume / Issue: 12 (1) Sequence Number: 1814674 Start / End Page: 1 - 13 Identifier: ISSN: 2000-2297
CoNE: https://pure.mpg.de/cone/journals/resource/2000-2297