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  NOD2 influences trajectories of intestinal microbiota recovery after antibiotic perturbation

Moltzau Anderson, J., Lipinski, S., Sommer, F., Pan, W.-H., Boulard, O., Rehman, A., et al. (2020). NOD2 influences trajectories of intestinal microbiota recovery after antibiotic perturbation. Cellular and Molecular Gastroenterology and Hepatology, 10(2), 365-389. Retrieved from http://www.sciencedirect.com/science/article/pii/S2352345X20300448.

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1-s2.0-S2352345X20300448-main.pdf (Publisher version), 8MB
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 Creators:
Moltzau Anderson, Jacqueline1, Author              
Lipinski, Simone, Author
Sommer, Felix, Author
Pan, Wei-Hung, Author
Boulard, Olivier, Author
Rehman, Ateequr, Author
Falk-Paulsen, Maren, Author
Stengel, Stephanie T., Author
Aden, Konrad, Author
Häsler, Robert, Author
Bharti, Richa, Author
Künzel, Sven2, Author              
Baines, John F.3, Author              
Chamaillard, Mathias, Author
Rosenstiel, Philip, Author
Affiliations:
1IMPRS for Evolutionary Biology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445639              
2Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              
3Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              

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Free keywords: Antibiotic Treatment, NOD2, IBD, Microbial Resilience
 Abstract: Background & Aims Loss-of-function variants in nucleotide-binding oligomerization domain-containing protein 2 (NOD2) impair the recognition of the bacterial cell wall component muramyl-dipeptide and are associated with an increased risk for developing Crohn’s disease. Likewise, exposure to antibiotics increases the individual risk for developing inflammatory bowel disease. Here, we studied the long-term impact of NOD2 on the ability of the gut bacterial and fungal microbiota to recover after antibiotic treatment. Methods Two cohorts of 20-week-old and 52-week-old wild-type (WT) C57BL/6J and NOD2 knockout (Nod2-KO) mice were treated with broad-spectrum antibiotics and fecal samples were collected to investigate temporal dynamics of the intestinal microbiota (bacteria and fungi) using 16S ribosomal RNA and internal transcribed spacer 1 sequencing. In addition, 2 sets of germ-free WT mice were colonized with either WT or Nod2-KO after antibiotic donor microbiota and the severity of intestinal inflammation was monitored in the colonized mice. Results Antibiotic exposure caused long-term shifts in the bacterial and fungal community composition. Genetic ablation of NOD2 was associated with delayed body weight gain after antibiotic treatment and an impaired recovery of the bacterial gut microbiota. Transfer of the postantibiotic fecal microbiota of Nod2-KO mice induced an intestinal inflammatory response in the colons of germ-free recipient mice compared with respective microbiota from WT controls based on histopathology and gene expression analyses. Conclusions Our data show that the bacterial sensor NOD2 contributes to intestinal microbial community composition after antibiotic treatment and may add to the explanation of how defects in the NOD2 signaling pathway are involved in the etiology of Crohn’s disease.

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Language(s): eng - English
 Dates: 2018-05-232020-03-312020-04-112020
 Publication Status: Published in print
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Title: Cellular and Molecular Gastroenterology and Hepatology
  Other : CMGH Journal
  Abbreviation : Cell Mol Gastroenterol Hepatol
Source Genre: Journal
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Publ. Info: United States : Elsevier
Pages: - Volume / Issue: 10 (2) Sequence Number: - Start / End Page: 365 - 389 Identifier: Other: http://v2.sherpa.ac.uk/id/publication/31048
Other: 2819778-1
Other: 2352-345X
CoNE: https://pure.mpg.de/cone/journals/resource/2352-345X