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  Label-freemonitoring and manipulation of microfluidic water-in-oil droplets

Frey, C., Pfeil, J., Neckernuss, T., Geiger, D., Weishaupt, K., Platzman, I., et al. (2020). Label-freemonitoring and manipulation of microfluidic water-in-oil droplets. View, 1(4): 20200101, pp. 1-11. doi:10.1002/VIW.20200101.

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 Creators:
Frey, Christoph1, Author              
Pfeil, Jonas, Author
Neckernuss, Tobias, Author
Geiger, Daniel, Author
Weishaupt, Klaus1, Author              
Platzman, Ilia1, Author              
Marti, Othmar, Author
Spatz, Joachim P.1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: active droplet manipulation; droplet‐based microfluidics; high‐throughput; label‐free; real‐time observation
 Abstract: Droplet‐based microfluidic technology offers several benefits: the integration of multiple laboratory functions into a single microfabricated chip, manual intervention possibilities, minimal sample consumption, and increased analysis speed and data precision. These advantages have boosted the widespread application of this technology in biological and biomedical research. Despite recent progress, considerable challenges remain to be addressed for end‐user applications. This especially concerns the difficulty of creating powerful and easy to implement methods for real‐time analysis and active manipulation of passing droplets. Toward this end, we developed a very sensitive optical device equipped with smart algorithms for real‐time label‐free monitoring and active manipulation of passing droplets. We demonstrate the advanced properties of the developed optical device by measuring different droplet production parameters as well as the label‐free detection of cells in droplets. Moreover, the newly developed technology was connected with a function generator system to allow for subsequent manipulation of the monitored droplets based on the measured parameters. As an example, we performed electric field‐mediated, label‐free sorting of cell‐containing droplets from empty ones. Furthermore, we achieved an efficient size‐based separation of droplets. We envision that the developed optical device will be a useful tool for the online monitoring of passing droplets and will be implemented for the integration and automation of various droplet‐based microfluidic functional units.

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Language(s): eng - English
 Dates: 2020-08-042020-06-062020-08-192020-10-102020-12-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/VIW.20200101
 Degree: -

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Title: View
Source Genre: Journal
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Publ. Info: Hoboken, NJ : Wiley
Pages: - Volume / Issue: 1 (4) Sequence Number: 20200101 Start / End Page: 1 - 11 Identifier: ISSN: 2688-268X