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  Metabolic Heterogeneity of Human Hepatocellular Carcinoma (HCC): Implications for Personalized Pharmacological Treatment

Berndt, N., Eckstein, J., Heucke, N., Wuensch, T., Gajowski, R., Stockmann, M., et al. (2020). Metabolic Heterogeneity of Human Hepatocellular Carcinoma (HCC): Implications for Personalized Pharmacological Treatment. The FEBS Journal, 2020: 15587. doi:10.1111/febs.15587.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-4B7A-D Version Permalink: http://hdl.handle.net/21.11116/0000-0007-4B7B-C
Genre: Journal Article

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 Creators:
Berndt, Nikolaus, Author
Eckstein, Johannes, Author
Heucke, Niklas , Author
Wuensch, Tilo, Author
Gajowski, Robert1, 2, Author              
Stockmann, Martin, Author
Meierhofer, David1, Author              
Holzhütter, Hermann-Georg , Author
Affiliations:
1Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479669              
2Department of Biology, Chemistry, and Pharmacy, Freie Universit ¨ at Berlin, GermanyOpen access funding enabled and organized by ProjektDEAL, ou_persistent22              

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Free keywords: kinetic modeling; liver; mathematical mode l; metabolism; tumor metabolism
 Abstract: Metabolic reprogramming is a characteristic feature of cancer cells, but there is no unique metabolic program for all tumors. Genetic and gene expression studies have revealed heterogeneous inter‐ and intratumor patterns of metabolic enzymes and membrane transporters. The functional implications of this heterogeneity remain often elusive. Here, we applied a systems biology approach to gain a comprehensive and quantitative picture of metabolic changes in individual hepatocellular carcinoma (HCC). We used protein intensity profiles determined by mass spectrometry in samples of 10 human HCCs and the adjacent noncancerous tissue to calibrate Hepatokin1, a complex mathematical model of liver metabolism. We computed the 24‐h profile of 18 metabolic functions related to carbohydrate, lipid, and nitrogen metabolism. There was a general tendency among the tumors toward downregulated glucose uptake and glucose release albeit with large intertumor variability. This finding calls into question that the Warburg effect dictates the metabolic phenotype of HCC. All tumors comprised elevated β‐oxidation rates. Urea synthesis was found to be consistently downregulated but without compromising the tumor's capacity for ammonia detoxification owing to increased glutamine synthesis. The largest intertumor heterogeneity was found for the uptake and release of lactate and the size of the cellular glycogen content. In line with the observed metabolic heterogeneity, the individual HCCs differed largely in their vulnerability against pharmacological treatment with metformin. Taken together, our approach provided a comprehensive and quantitative characterization of HCC metabolism that may pave the way for a computational a priori assessment of pharmacological therapies targeting metabolic processes of HCC.

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Language(s): eng - English
 Dates: 2020-10-052020-10-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1111/febs.15587
 Degree: -

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Title: The FEBS Journal
  Other : The Federation if European Biochemical Societies Journal
Source Genre: Journal
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Publ. Info: Wiley-Blackwell
Pages: - Volume / Issue: 2020 Sequence Number: 15587 Start / End Page: - Identifier: ISSN: 1742-464X
CoNE: https://pure.mpg.de/cone/journals/resource/954925398485