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  Photoactivatable fluorophore for stimulated emission depletion (STED) microscopy and bioconjugation technique for hydrophobic labels

Weber, M., Khan, T. A., Patalag, L. J., Bossi, M., Leutenegger, M., Belov, V. N., et al. (2021). Photoactivatable fluorophore for stimulated emission depletion (STED) microscopy and bioconjugation technique for hydrophobic labels. Chemistry – A European Journal, 27(1), 451-458. doi:10.1002/chem.202004645.

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 Creators:
Weber, Michael, Author
Khan, Taukeer A., Author
Patalag, Lukas J., Author
Bossi, Mariano1, Author              
Leutenegger, Marcel, Author
Belov, Vladimir N., Author
Hell, Stefan W.1, Author              
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: fluorescence, photoactivation, stimulated emission depletion (STED), optical superresolution, protein labelling, immunofluorescence
 Abstract: The use of photoactivatable dyes in STED microscopy has so far been limited by two—photon activation through the STED beam and by the fact that photoactivatable dyes are poorly solvable in water. Here we report ONB‐2SiR, a fluorophore that can be both photoactivated in the UV and specifically de‐excited by STED at 775 nm. Likewise, we introduce a conjugation and purification protocol to effectively label primary and secondary antibodies with moderately water‐soluble dyes. Greatly reducing dye aggregation, our technique provides a defined and tunable degree of labeling, and improves the imaging performance of dye conjugates in general.

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Language(s): eng - English
 Dates: 2020-10-212020-10-212020-10-232021-01-04
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Chemistry – A European Journal
  Other : Chem. – Eur. J.
  Other : Chem. Eur. J.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 27 (1) Sequence Number: - Start / End Page: 451 - 458 Identifier: ISSN: 0947-6539
CoNE: https://pure.mpg.de/cone/journals/resource/954926979058