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  Quantitative and simultaneous measurement of oxygen consumption rates in rat brain and skeletal muscle using 17 O MRS imaging at 16.4T

Wiesner, H., Balla, D., Scheffler, K., Ugurbil, K., Zhu, X.-H., Chen, W., et al. (2020). Quantitative and simultaneous measurement of oxygen consumption rates in rat brain and skeletal muscle using 17 O MRS imaging at 16.4T. Magnetic Resonance in Medicine, Epub ahead. doi:10.1002/mrm.28552.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-4DDA-E Version Permalink: http://hdl.handle.net/21.11116/0000-0007-4DDB-D
Genre: Journal Article

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Wiesner, HM1, 2, Author              
Balla, DZ1, 2, Author              
Scheffler, K1, 2, Author              
Ugurbil, K, Author
Zhu, X-H, Author
Chen , W, Author
Uludag, K, Author              
Pohmann, R1, 2, Author              
Affiliations:
1Department High-Field Magnetic Resonance, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497796              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

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 Abstract: Purpose: Oxygen-17 (17 O) MRS imaging, successfully used in the brain, is extended by imaging the oxygen metabolic rate in the resting skeletal muscle and used to determine the total whole-body oxygen metabolic rate in the rat. Methods: During and after inhalations of 17 O2 gas, dynamic 17 O MRSI was performed in rats (n = 8) ventilated with N2 O or N2 at 16.4 T. Time courses of the H2 17 O concentration from regions of interest located in brain and muscle tissue were examined and used to fit an animal-adapted 3-phase metabolic model of oxygen consumption. CBF was determined with an independent washout method. Finally, body oxygen metabolic rate was calculated using a global steady-state approach. Results: Cerebral metabolic rate of oxygen consumption was 1.97 ± 0.19 μmol/g/min on average. The resting metabolic rate of oxygen consumption in skeletal muscle was 0.32 ± 0.12 μmol/g/min and >6 times lower than cerebral metabolic rate of oxygen consumption. Global oxygen consumed by the body was 24.2 ± 3.6 mL O2 /kg body weight/min. CBF was estimated to be 0.28 ± 0.02 mL/g/min and 0.34 ± 0.06 mL/g/min for the N2 and N2 O ventilation condition, respectively. Conclusion: We have evaluated the feasibility of 17 O MRSI for imaging and quantifying the oxygen consumption rate in low metabolizing organs such as the skeletal muscle at rest. Additionally, we have shown that CBF is slightly increased in the case of ventilation with N2 O. We expect this study to be beneficial to the application of 17 O MRSI to a wider range of organs, although further validation is advised.

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 Dates: 2020-10
 Publication Status: Published online
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 Identifiers: DOI: 10.1002/mrm.28552
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Title: Magnetic Resonance in Medicine
Source Genre: Journal
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Publ. Info: New York : Wiley-Liss
Pages: - Volume / Issue: Epub ahead Sequence Number: - Start / End Page: - Identifier: ISSN: 0740-3194
CoNE: https://pure.mpg.de/cone/journals/resource/954925538149