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  Reprogramming competence of OCT factors is determined by transactivation domains

Kim, K.-P., Wu, Y., Yoon, J., Adachi, K., Wu, G., Velychko, S., et al. (2020). Reprogramming competence of OCT factors is determined by transactivation domains. SCIENCE ADVANCES, 6(36): eaaz7364. doi:10.1126/sciadv.aaz7364.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-4E99-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-4E9A-5
Genre: Journal Article

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 Creators:
Kim, Kee-Pyo, Author
Wu, You, Author
Yoon, Juyong, Author
Adachi, Kenjiro, Author
Wu, Guangming, Author
Velychko, Sergiy, Author
Maccarthy, Caitlin M., Author
Shin, Borami, Author
Roepke, Albrecht, Author
Arauzo-Bravo, Marcos J., Author
Stehling, Martin, Author
Han, Dong Wook, Author
Gao, Yawei, Author
Kim, Johnny1, Author              
Gao, Shaorong, Author
Schoeler, Hans R., Author
Affiliations:
1Cardiac Development and Remodeling, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591695              

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 Abstract: OCT4 (also known as POU5F1) plays an essential role in reprogramming. It is the only member of the POU (Pit-Oct-Unc) family of transcription factors that can induce pluripotency despite sharing high structural similarities to all other members. Here, we discover that OCT6 (also known as POU3F1) can elicit reprogramming specifically in human cells. OCT6-based reprogramming does not alter the mesenchymal-epithelial transition but is attenuated through the delayed activation of the pluripotency network in comparison with OCT4-based reprogramming. Creating a series of reciprocal domain-swapped chimeras and mutants across all OCT factors, we clearly delineate essential elements of OCT4/OCT6-dependent reprogramming and, conversely, identify the features that prevent induction of pluripotency by other OCT factors. With this strategy, we further discover various chimeric proteins that are superior to OCT4 in reprogramming. Our findings clarify how reprogramming competences of OCT factors are conferred through their structural components.

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 Dates: 2020-09-02
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000567766700003
DOI: 10.1126/sciadv.aaz7364
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Title: SCIENCE ADVANCES
Source Genre: Journal
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Pages: - Volume / Issue: 6 (36) Sequence Number: eaaz7364 Start / End Page: - Identifier: ISSN: 2375-2548