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  Precipitation of calcium carbonate inside giant unilamellar vesicles composed of fluid-phase lipids

Witt, H., Yandrapalli, N., Sari, M., Turco, L., Robinson, T., & Steinem, C. (2020). Precipitation of calcium carbonate inside giant unilamellar vesicles composed of fluid-phase lipids. Langmuir, 36(44), 13244-13250. doi:10.1021/acs.langmuir.0c02175.

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 Creators:
Witt, Hannes, Author
Yandrapalli, Naresh1, Author              
Sari, Merve, Author
Turco, Laura, Author
Robinson, Tom1, Author              
Steinem, Claudia, Author
Affiliations:
1Tom Robinson, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2288691              

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Free keywords: Calcium; Vesicles; Lipids; Membranes; Inorganic carbon compounds
 Abstract: Biomineralization of CaCO3 commonly involves the formation of amorphous CaCO3 precursor particles that are produced in a confined space surrounded by a lipid bilayer. While the influence of confinement itself has been investigated with different model systems, the impact of an enclosing continuous lipid bilayer on CaCO3 formation in a confined space is still poorly understood as appropriate model systems are rare. Here, we present a new versatile method based on droplet-based microfluidics to produce fluid-phase giant unilamellar vesicles (GUVs) in the presence of high CaCl2 concentrations. These GUVs can be readily investigated by means of confocal laser scanning microscopy in combination with bright-field microscopy, demonstrating that the formed CaCO3 particles are in conformal contact with the fluid-phase lipid bilayer and thus suggesting a strong interaction between the particle and the membrane. Atomic force microscopy adhesion studies with membrane-coated spheres on different CaCO3 crystals corroborated this notion of a strong interaction between the lipids and CaCO3.

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Language(s): eng - English
 Dates: 2020-10-282020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1021/acs.langmuir.0c02175
BibTex Citekey: doi:10.1021/acs.langmuir.0c02175
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Title: Langmuir
  Abbreviation : Langmuir
Source Genre: Journal
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Publ. Info: Columbus, OH : American Chemical Society
Pages: - Volume / Issue: 36 (44) Sequence Number: - Start / End Page: 13244 - 13250 Identifier: ISSN: 0743-7463