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  Surface Co-presentation of BMP-2 and integrin selective ligands at the nanoscale favors α5β1 integrin-mediated adhesion

Posa, F., Baha-Schwab, E. H., Wei, Q., Benedetto, A. D., Neubauer, S., Reichart, F., et al. (2021). Surface Co-presentation of BMP-2 and integrin selective ligands at the nanoscale favors α5β1 integrin-mediated adhesion. Biomaterials, 267: 120484, pp. 1-15. doi:10.1016/j.biomaterials.2020.120484.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-518D-F Version Permalink: http://hdl.handle.net/21.11116/0000-0007-518E-E
Genre: Journal Article
Alternative Title : Surface Co-presentation of BMP-2 and integrin selective ligands at the nanoscale favors alpha5beta1 integrin-mediated adhesion

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Biomaterials_267_2021_120484.pdf (Any fulltext), 12MB
 
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 Creators:
Posa, Francesca1, Author              
Baha-Schwab, Elisabeth H.1, Author              
Wei, Qiang1, Author              
Benedetto, Adriana Di, Author
Neubauer, Stefanie, Author
Reichart, Florian, Author
Kessler, Horst, Author
Spatz, Joachim P.1, Author              
Albigez-Rizo, Corinne, Author
Mori, Giorgio, Author
Cavalcanti-Adam, Elisabetta Ada1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: BMP-2; Integrins; Focal adhesions; Surface functionalization; Click chemistry
 Abstract: Here we present the use of surface nanopatterning of covalently immobilized BMP-2 and integrin selective ligands to determine the specificity of their interactions in regulating cell adhesion and focal adhesion assembly. Gold nanoparticle arrays carrying single BMP-2 dimers are prepared by block-copolymer micellar nanolithography and azide-functionalized integrin ligands (cyclic-RGD peptides or α5β1 integrin peptidomimetics) are immobilized on the surrounding polyethylene glycol alkyne by click chemistry. Compared to BMP-2 added to the media, surface immobilized BMP-2 (iBMP-2) favors the spatial segregation of adhesion clusters and enhances focal adhesion (FA) size in cells adhering to α5β1 integrin selective ligands. Moreover, iBMP-2 copresented with α5β1 integrin ligands induces the recruitment of αvβ3 integrins in FAs. When copresented with RGD, iBMP-2 induces the assembly of a higher number of FAs, which are not affected by α5β1 integrin blocking. Our dual-functionalized platforms offer the possibility to study the crosstalk between integrins and BMP receptors, and more in general they could be used to address the spatial regulation of growth factors and adhesion receptors crosstalk on biomimetic surfaces.

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Language(s): eng - English
 Dates: 2020-10-142020-05-052020-10-212020-10-262021-01-01
 Publication Status: Published in print
 Pages: -
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 Rev. Type: Peer
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Title: Biomaterials
Source Genre: Journal
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Publ. Info: Guildford, England : Elsevier
Pages: - Volume / Issue: 267 Sequence Number: 120484 Start / End Page: 1 - 15 Identifier: ISSN: 0142-9612
CoNE: https://pure.mpg.de/cone/journals/resource/954925472369