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  Signal-enhanced real-time magnetic resonance of enzymatic reactions at millitesla fields

Korchak, S., Jagtap, A., & Glöggler, S. (2021). Signal-enhanced real-time magnetic resonance of enzymatic reactions at millitesla fields. Chemical Science, 12(1), 314-319. doi:10.1039/D0SC04884D.

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Korchak, S.1, Author              
Jagtap, A.1, Author              
Glöggler, S.1, Author              
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1Research Group of NMR Signal Enhancement, MPI for Biophysical Chemistry, Max Planck Society, ou_2396691              

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 Abstract: The phenomenon of nuclear magnetic resonance (NMR) is widely applied in biomedical and biological science to study structures and dynamics of proteins and their reactions. Despite its impact, NMR is an inherently insensitive phenomenon and has driven the field to construct spectrometers with increasingly higher magnetic fields leading to more detection sensitivity. Here, we are demonstrating that enzymatic reactions can be followed in real-time at millitesla fields, three orders of magnitude lower than the field of state-of-the-art NMR spectrometers. This requires signal-enhancing samples via hyperpolarization. Within seconds, we have enhanced the signals of 2-13C-pyruvate, an important metabolite to probe cancer metabolism, in 22 mM concentrations (up to 10.1% ± 0.1% polarization) and show that such a large signal allows for the real-time detection of enzymatic conversion of pyruvate to lactate at 24 mT. This development paves the pathways for biological studies in portable and affordable NMR systems with a potential for medical diagnostics.

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Language(s): eng - English
 Dates: 2020-10-302021-01-07
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1039/D0SC04884D
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Title: Chemical Science
Source Genre: Journal
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Pages: - Volume / Issue: 12 (1) Sequence Number: - Start / End Page: 314 - 319 Identifier: -