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  Crystallization and Structure of the Photosynthetic Reaction Centres from Rhodobacter sphaeroides - wild type and mutants

Fritzsch, G., Ermler, U., Merckel, M. C., & Michel, H. (1996). Crystallization and Structure of the Photosynthetic Reaction Centres from Rhodobacter sphaeroides - wild type and mutants. In M.-E. Michel-Beyerle (Ed.), The Reaction Center of Photosynthetic Bacteria (pp. 3-13). Berlin, Heidelberg: Springer.

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 Creators:
Fritzsch, Günter1, Author           
Ermler, Ulrich1, Author           
Merckel, Michael C.1, Author           
Michel, Hartmut1, Author           
Affiliations:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: Photosynthesis; reaction centre; crystallization; structure
 Abstract: Trigonal crystals of the photosynthetic reaction centres from the purple bacterium Rhodobacter sphaeroides have been grown with potassium phosphate as precipitant. These crystals diffract to 2.6 Å and are suitable for a detailed structure determination. The cofactor binding is similar to that observed in the reaction centres of Rhodopseudomonas viridis. A 23 Å long water chain connects the secondary quinone QB with the cytoplasm. The structures of the mutants Thr M222 → Val, Trp M252 → Phe, and Trp M252 → Tyr show minor differences compared with the wild type. In the carotenoidless strain R26 the carotenoid-binding site is occupied by a detergent molecule.

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Language(s): eng - English
 Dates: 1996
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-3-642-61157-5_1
 Degree: -

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Title: The Reaction Center of Photosynthetic Bacteria
  Subtitle : Structure and Dynamics
Source Genre: Book
 Creator(s):
Michel-Beyerle, Maria-Elisabeth 1, Editor
Affiliations:
1 Institut für Physikalische und Theoretische Chemie, Technische Universität München, Garching, Germany, ou_persistent22            
Publ. Info: Berlin, Heidelberg : Springer
Pages: 426 Volume / Issue: - Sequence Number: - Start / End Page: 3 - 13 Identifier: ISBN: 978-3-642-64723-9
ISBN: 978-3-642-61157-5