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  Identification and Characterization of the Heat-Induced Plastidial Stress Granules Reveal New Insight Into Arabidopsis Stress Response

Chodasiewicz, M., Sokolowska, E., Nelson-Dittrich, A. C., Masiuk, A., Moreno, J., Nelson, A. D. L., et al. (2020). Identification and Characterization of the Heat-Induced Plastidial Stress Granules Reveal New Insight Into Arabidopsis Stress Response. Frontiers in Plant Science, 11: 597592. doi:10.3389/fpls.2020.595792.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-5AE5-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-5AE6-1
Genre: Journal Article

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Chodasiewicz, M.1, Author              
Sokolowska, E.2, Author              
Nelson-Dittrich, Anna C.3, Author
Masiuk, Aleksandra3, Author
Moreno, J.C.4, Author              
Nelson, Andrew D. L.3, Author
Skirycz, A.5, Author              
Affiliations:
1Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              
2Intercellular Macromolecular Transport, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753331              
3External Organizations, ou_persistent22              
4Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
5Small-Molecule Signalling, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2586692              

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 Abstract: Plants exhibit different physiological and molecular responses to adverse changes in their environment. One such molecular response is the sequestration of proteins, RNAs, and metabolites into cytoplasmic bodies called stress granules (cSGs). Here we report that, in addition to cSGs, heat stress also induces the formation of SG-like foci (cGs) in the chloroplasts of the model plant Arabidopsis thaliana. Similarly to the cSGs, (i) cpSG assemble rapidly in response to stress and disappear when the stress ceases, (ii) cpSG formation is inhibited by treatment with a translation inhibitor (lincomycin), and (iii) cpSG are composed of a stable core and a fluid outer shell. A previously published protocol for cSG extraction was successfully adapted to isolate cpSG, followed by protein, metabolite, and RNA analysis. Analogously to the cSGs, cpSG sequester proteins essential for SG formation, dynamics, and function, also including RNA-binding proteins with prion-like domain, ATPases and chaperones, and the amino acids proline and glutamic acid. However, the most intriguing observation relates to the cpSG localization of proteins, such as a complete magnesium chelatase complex, which is involved in photosynthetic acclimation to stress. These data suggest that cpSG have a role in plant stress tolerance.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: DOI: 10.3389/fpls.2020.595792
BibTex Citekey: 10.3389/fpls.2020.595792
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Title: Frontiers in Plant Science
  Abbreviation : Front. Plant Sci.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 11 Sequence Number: 597592 Start / End Page: - Identifier: ISSN: 1664-462X
CoNE: https://pure.mpg.de/cone/journals/resource/1664462X