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  A systematic investigation of human DNA preservation in medieval skeletons

Parker, E. C., Rohrlach, A. B., Friederich, S., Nagel, S., Meyer, M., Krause, J., et al. (2020). A systematic investigation of human DNA preservation in medieval skeletons. Scientific Reports, 10(1): 18225. doi:10.1038/s41598-020-75163-w.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-5B50-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-77D3-5
Genre: Journal Article

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Supplementary Information 1. (Supplementary material)
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(last seen Nov. 2020)
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Supplementary Information 2. (Supplementary material)
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(last seen Nov. 2020)

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 Creators:
Parker, Edward Cody1, Author              
Rohrlach, Adam Ben1, Author              
Friederich, Susanne, Author
Nagel, Sarah, Author              
Meyer, Matthias, Author              
Krause, Johannes1, 2, Author              
Bos, Kirsten I.1, Author              
Haak, Wolfgang1, Author              
Affiliations:
1Archaeogenetics, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074310              
2MHAAM, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2541699              

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Free keywords: Biological anthropology, High-throughput screening, Population genetics
 Abstract: Ancient DNA (aDNA) analyses necessitate the destructive sampling of archaeological material. Currently, the cochlea, part of the osseous inner ear located inside the petrous pyramid, is the most sought after skeletal element for molecular analyses of ancient humans as it has been shown to yield high amounts of endogenous DNA. However, destructive sampling of the petrous pyramid may not always be possible, particularly in cases where preservation of skeletal morphology is of top priority. To investigate alternatives, we present a survey of human aDNA preservation for each of ten skeletal elements in a skeletal collection from Medieval Germany. Through comparison of human DNA content and quality we confirm best performance of the petrous pyramid and identify seven additional sampling locations across four skeletal elements that yield adequate aDNA for most applications in human palaeogenetics. Our study provides a better perspective on DNA preservation across the human skeleton and takes a further step toward the more responsible use of ancient materials in human aDNA studies.

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Language(s): eng - English
 Dates: 2020-10-26
 Publication Status: Published online
 Pages: 16
 Publishing info: -
 Table of Contents: Introduction
Results
Discussion
Conclusions
Methods
- Sample selection, pre‑treatment, and bone powder generation
- DNA extraction, library preparation, and sequencing
- Evaluation criteria
- Contamination estimates
- Mapping
- Calculations
- Mixed effects modelling
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41598-020-75163-w
Other: shh2761
 Degree: -

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Project name : PALEoRIDER
Grant ID : 771234
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : CoDisEASe
Grant ID : 805268
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

Source 1

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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 10 (1) Sequence Number: 18225 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322