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  STM of metal embedded and coated DNA and DNA–protein complexes

Müller-Reichert, T., Butt, H.-J., & Gross, H. (1996). STM of metal embedded and coated DNA and DNA–protein complexes. Journal of Microscopy, 182(3), 169-176. doi:10.1046/j.1365-2818.1996.62426.x.

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 Creators:
Müller-Reichert, T.1, Author
Butt, Hans-Jürgen2, Author              
Gross, H., Author
Affiliations:
1Eidgenössische Technische Hochschule, Institut für Zellbiologie, ETH‐Hönggerberg, CH‐8093 Zürich, Switzerland, ou_persistent22              
2Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

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Free keywords: DNA–protein interaction; DNA; RAP1; SFM; STM; TEM
 Abstract: Bare and Pt/Ir/C‐coated DNA has been analysed using scanning tunnelling microscopy (STM). To achieve reproducible imaging of bare DNA on mica ethanol/air‐dried molecules were embedded in Pt/C. By peeling the metal film off the mica, the previously mica‐exposed side of the Pt/C‐film with the embedded DNA molecules was accessible for STM analysis. By applying this replica/anchoring technique only hollow trenches in the metal film, and not the DNA itself, could be visualized. The gaps averaged 3.1 nm (± 0.9 nm) wide and 1 nm (± 0.5 nm) deep. Using scanning force microscopy it could be confirmed that the DNA remained in the Pt/C film during the peel‐off procedure. For STM, DNA fragments were also coated with 0.7–1 nm Pt/Ir/C. Owing to the high Z‐resolution the STM samples were coated at a high elevation angle (65°), thereby minimizing the problem of self‐shadowing. Coating by Pt/Ir/C allowed routine imaging and quantitative analysis of both ethanol/air‐ and freeze‐dried DNA under atmospheric conditions. After ethanol/air drying measured values for DNA width and height were 5.1 nm (± 1.8 nm) and 0.9 nm (± 0.2 nm), respectively. Freeze‐dried DNA averaged 4.2 nm (± 1.3 nm) wide and 1.1 nm (± 0.1 nm) high. A Pt/Ir/C‐coating was also applied to visualize DNA–protein interaction using STM

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Language(s): eng - English
 Dates: 1995-08-111996-02-072003-10-311996-06-01
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1046/j.1365-2818.1996.62426.x
 Degree: -

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Title: Journal of Microscopy
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 182 (3) Sequence Number: - Start / End Page: 169 - 176 Identifier: ISSN: 0022-2720
CoNE: https://pure.mpg.de/cone/journals/resource/954927663105_2