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  Prediction of the Structure of an Integral Membrane Protein: The Light-Harvesting Complex II of Rhodospirillum molischianum

Hu, X., Xu, D., Hamer, K., Schulten, K., Koepke, J., & Michel, H. (1996). Prediction of the Structure of an Integral Membrane Protein: The Light-Harvesting Complex II of Rhodospirillum molischianum. In K. M. Merz, Jr. (Ed.), Biological Membranes (pp. 503-533). Boston, USA: Birkhäuser.

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 Creators:
Hu, Xiche1, Author
Xu , Dong1, Author
Hamer, Kenneth1, Author
Schulten, Klaus1, Author
Koepke, Jürgen2, Author           
Michel, Hartmut2, Author                 
Affiliations:
1Theoretical Biophysics, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA, ou_persistent22              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: Integral Membrane Protein; Transmembrane Helix; Transmembrane Segment; Molecular Replacement; Resonance Raman Spectrum
 Abstract: We illustrate in this chapter how one proceeds to predict the structure of integral membrane proteins when a highly homologous structure is unknown. We focus here on the prediction of the structure of the light-harvesting complex II (LH-II) of Rhodospirillum molischianum, an integral membrane protein of 16 polypeptides aggregating and binding to 24 bacteriochlorophyll a’s and 12 lycopenes. Hydropathy analysis was performed to identify the putative transmembrane segments, which were independently verified by multiple sequence alignment propensity analyses and homology modeling. A consensus assignment for secondary structure was derived from a combination of all the prediction methods used. Transmembrane helices were built by comparative modeling. The resulting tertiary structures were then aggregated into a quaternary structure through molecular dynamics simulations and energy minimization under constraints provided by site directed mutagenesis and FT Resonance Raman spectra, as well as conservation of residues. The structure of LH-II, so determined, was an octamer of αβ heterodimers forming a ring with a diameter of 70 Å. We discuss how the resulting structure may be used to solve the phase problem in X-ray crystallography in a procedure called molecular replacement. We will also discuss the exciton structure which results from the circular arrangement of chlorophyls in LH-II.

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Language(s): eng - English
 Dates: 1996
 Publication Status: Issued
 Pages: 31
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-4684-8580-6
 Degree: -

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Title: Biological Membranes
  Subtitle : A Molecular Perspective from Computation and Experiment
Source Genre: Book
 Creator(s):
Merz, Jr., Kenneth M. 1, Editor
Roux, Benoît2, Author
Affiliations:
1 Department of Chemistry, Pennsylvania State University, University Park, USA, ou_persistent22            
2 Groupe de Recherche en Transport Membranaire (GRTM), Départements de physique et de chimie, Université de Montréal, Montréal, Canada, ou_persistent22            
Publ. Info: Boston, USA : Birkhäuser
Pages: 592 Volume / Issue: - Sequence Number: - Start / End Page: 503 - 533 Identifier: ISBN: 978-1-4684-8582-0
ISBN: 978-1-4684-8580-6