ausblenden:
Schlagwörter:
automated electron tomography; transmission electron microscopy;
low-dose microscopy; three-dimensional reconstruction; triad junction;
IVEM
Zusammenfassung:
Low-dose automated tomography has been implemented on a 400-kV JEOL intermediate voltage electron microscope. Instrumentation and procedures for automatic tomographic series data collection are described. Difficulties encountered and ways to overcome them are discussed. A low-dose tomographic projection series of a triad junction of frog sartorius muscle was semiautomatically collected and a 3-D reconstruction of this organelle was made.
