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  Single-allele chromatin interactions identify regulatory hubs in dynamic compartmentalized domains

Oudelaar, A. M., Davies, J. O. J., Hanssen, L. L. P., Telenius, J. M., Schwessinger, R., Liu, Y., et al. (2018). Single-allele chromatin interactions identify regulatory hubs in dynamic compartmentalized domains. Nature Genetics, 50(12), 1744-1751. doi:10.1038/s41588-018-0253-2.

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 Creators:
Oudelaar, A. M.1, Author           
Davies, J. O. J., Author
Hanssen, L. L. P., Author
Telenius, J. M., Author
Schwessinger, R., Author
Liu, Y., Author
Brown, J. M., Author
Downes, D. J., Author
Chiariello, A. M., Author
Bianco, S., Author
Nicodemi, M., Author
Buckle, V. J., Author
Dekker, J., Author
Higgs, D. R., Author
Hughes, J. R., Author
Affiliations:
1Lise Meitner Group Genome Organization and Regulation, MPI for Biophysical Chemistry, Max Planck Society, ou_3261271              

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 Abstract: The promoters of mammalian genes are commonly regulated by multiple distal enhancers, which physically interact within discrete chromatin domains. How such domains form and how the regulatory elements within them interact in single cells is not understood. To address this we developed Tri-C, a new chromosome conformation capture (3C) approach, to characterize concurrent chromatin interactions at individual alleles. Analysis by Tri-C identifies heterogeneous patterns of single-allele interactions between CTCF boundary elements, indicating that the formation of chromatin domains likely results from a dynamic process. Within these domains, we observe specific higher-order structures that involve simultaneous interactions between multiple enhancers and promoters. Such regulatory hubs provide a structural basis for understanding how multiple cis-regulatory elements act together to establish robust regulation of gene expression.

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Language(s): eng - English
 Dates: 2018-10-292018-12
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41588-018-0253-2
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Title: Nature Genetics
Source Genre: Journal
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Pages: - Volume / Issue: 50 (12) Sequence Number: - Start / End Page: 1744 - 1751 Identifier: -