English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Low-input Capture-C: A chromosome conformation capture assay to analyze chromatin architecture in small numbers of cells

Oudelaar, A. M., Downes, D. J., Davies, J. O. J., & Hughes, J. R. (2017). Low-input Capture-C: A chromosome conformation capture assay to analyze chromatin architecture in small numbers of cells. Bio-Protocol, 7(23): e2645. doi:10.21769/BioProtoc.2645.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Oudelaar, A. M.1, Author           
Downes, D. J., Author
Davies, J. O. J., Author
Hughes, J. R., Author
Affiliations:
1Lise Meitner Group Genome Organization and Regulation, MPI for Biophysical Chemistry, Max Planck Society, ou_3261271              

Content

show
hide
Free keywords: Nuclear organization, Chromatin architecture, Chromosome conformation capture (3C), Capture-C, Low input, Small cell numbers
 Abstract: Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.

Details

show
hide
Language(s): eng - English
 Dates: 2017-12-05
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.21769/BioProtoc.2645
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Bio-Protocol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 (23) Sequence Number: e2645 Start / End Page: - Identifier: -